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- W54285517 abstract "Publisher Summary This chapter discusses the isolation of spermatozoal antigens. The antigens involved in spermatozoal agglutination and immobilization are present on spermatozoa. In the guinea pig, spermatozoal autoantigens are found to be able to induce several types of immune reactions—delayed hypersensitivity, complement fixing and anaphylactic antibodies, and autoimmune aspermatogenic orchiepididymitis. Cyt ct is a poor immunogen; however, cross-linking involving glutaraldehyde treatment gives a product suitable for use. Antiserum to Cyt ct is treated by affinity chromatography to remove antibodies to cyt cs. By immunofluorescence, cyt ct is localized in mitochondria. It can be detected in primary spermatocytes at pachytene or at later cell stages but not in spermatogonia, though cyt cs can be detected in such cells. As transcription occurs for the last time in the primary spermatocyte, any component able to be isolated from spermatozoa can be found in the testis, and any protein in spermatozoa must be produced in the testis. In the guinea pig, antigens on spermatids are detectable also on secondary spermatocytes." @default.
- W54285517 created "2016-06-24" @default.
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- W54285517 date "1977-01-01" @default.
- W54285517 modified "2023-10-16" @default.
- W54285517 title "ISOLATION OF SPERMATOZOAL ANTIGENS" @default.
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- W54285517 doi "https://doi.org/10.1016/b978-0-7295-0006-7.50011-2" @default.
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