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- W54293655 abstract "This chapter focuses on a permeabilized cell system to study peroxisomal protein import. The development of permeabilized cell systems has facilitated the reconstitution and biochemical characterization of a variety of intracellular protein trafficking steps. In an experiment discussed in the chapter, coverslip-attached cells are permeabilized with streptolysin O (SLO) and incubated with exogenously added peroxisomal proteins. The protein import into peroxisomes is analyzed by a simple immunofluorescence technique. The import of proteins into peroxisomes is directed by peroxisomal targeting signals (PTS). A carboxy-terminal tripeptide with the sequence SKL represents a prototype PTS. However, treatment of cells with SLO, especially with higher SLO concentrations, significantly reduces cell attachment and cells are easily washed off the coverslips." @default.
- W54293655 created "2016-06-24" @default.
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- W54293655 date "1994-01-01" @default.
- W54293655 modified "2023-09-26" @default.
- W54293655 title "A Permeabilized Cell System to Study Peroxisomal Protein Import" @default.
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- W54293655 doi "https://doi.org/10.1016/b978-0-12-164717-9.50023-4" @default.
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