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- W54579470 abstract "Publisher Summary This chapter discusses the biological function and migratory behavior of T-lymphocytes mediating delayed-type hypersensitivity. In a study described in the chapter, (B6D2) F1 mice were injected with cyclophosphamide, and two days later, they were sensitized with 108 sheep red blood cells (SRBC). To obtain peripheral effector T cells, peritoneal exudates were induced on day 5 of the immune response with 2 ml of 5% casein. 48 h later, peritoneal exudate cells were collected and peritoneal exudate T lymphocytes (PEL) were enriched by nylon wool incubation. Afterward, PEL were treated in vitro and were washed. Then, 2 × 106-treated PEL were injected i.v. into normal recipient mice. The capacity of effector T cells to mediate DTH to SRBC was tested by challenging cell recipients with 1 × 108 SRBC given into one hind footpad and by measuring the degree of swelling 24 h later. It was found that treatment of PEL with 50 U/ml neuraminidase (NA) at 37°C for 1 h removed approximately 25% of total content of cellular sialic acids." @default.
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- W54579470 date "1979-01-01" @default.
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- W54579470 title "BIOLOGICAL FUNCTION AND MIGRATORY BEHAVIOUR OF T-LYMPHOCYTES MEDIATING DELAYED TYPE HYPERSENSITIVITY DEPEND ON THE OXIDATION/REDUCTION STATE OF MEMBRANE CARBOHYDRATES" @default.
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- W54579470 doi "https://doi.org/10.1016/b978-0-12-569650-0.50040-1" @default.
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