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- W5464457 abstract "Traditional methods like pruning and breeding have historically been used in crop production to divert photoassimilates to harvested organs, but molecular biotechnology is now poised to significantly increase yield by manipulating resource partitioning. It was hypothesized that metabolic engineering in targeted sink tissues can favor resource partitioning to increase harvest. Raffinose Family Oligosaccharides (RFOs) are naturally occurring oligosaccharides that are widespread in plants and are responsible for carbon transport, storage and protection against cold and drought stress. Transgenic plants (GRS47, GRS63) were engineered to generate and transport more RFOs through the phloem than the wild type plants. The transgenic lines produced more RFOs and the RFOs were also detected in their phloem exudates. But the 14CO2 labeling and subsequent thin layer chromatography analysis showed that the RFOs were most likely sequestered in an inactive pool and accumulate over time. Crossing GRS47 and GRS63 lines with MIPS1 plants (that produces more myo-inositol, a substrate in the RFO biosynthetic pathway) did not significantly increase the RFOs in the crossed lines. For future manipulation of RFO degradation in sink organs, the roles of the endogenous α-galactosidases were analyzed. The alkaline α-galactosidases (AtSIP1 and AtSIP2 in Arabidopsis) are most likely responsible for digesting RFOs in the cytoplasm and may influence the ability to manipulate RFO levels in engineered plants. Atsip1/2 (AtSIP1/AtSIP2 double-knockout plants) were generated and phenotypically characterized based on seed germination patterns, flowering time, and sugar content to observe the impact on RFO sugar levels. The observations and analysis from these lines provide a basis for further insight in the manipulation of resource allocation between source and sink tissues in plants for future research." @default.
- W5464457 created "2016-06-24" @default.
- W5464457 creator A5009661168 @default.
- W5464457 date "2013-08-01" @default.
- W5464457 modified "2023-09-23" @default.
- W5464457 title "Metabolic Engineering in Plants to Control Source/sink Relationship and Biomass Distribution" @default.
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