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- W55016310 abstract "Publisher Summary This chapter examines the quantitative methods for measuring cell growth and death. Cell growth and death can be investigated by microscopic observations of cells in monolayers. More precise measurements are achieved by cell counts performed by the means of a hemocytometer or by automatic electronic cell counters. Visual counting is usually accompanied by vital stains which distinguish viable cells from damaged or dead ones. The measurement of cell growth by the determination of cell protein concentration is a rapid and reliable method which can be employed in large-scale testing. The amount of cell growth can be determined with cultures grown in suspension and also with cultures adhering to a surface. The ease of determination of the initial protein concentration and of the subsequent concentrations during the time course of the experiment makes this method very attractive. With appropriate conversion factors, the values may be changed to dry weight, protein nitrogen, or cell count. This method has found great utility for assessing cytocidal and cytostatic effects of extrinsic substances and is also useful for monitoring the dynamics of cell populations during the logarithmic phase of growth." @default.
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- W55016310 date "1973-01-01" @default.
- W55016310 modified "2023-09-27" @default.
- W55016310 title "Quantitative Methods for Measuring Cell Growth and Death" @default.
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- W55016310 doi "https://doi.org/10.1016/b978-0-12-427150-0.50102-9" @default.
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