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- W55405400 abstract "In this chapter, we describe a gene-specific quantitative PCR (QPCR)-based assay for the measurement of DNA damage, using amplification of long DNA targets. This assay has been used extensively to measure the integrity of both nuclear and mitochondrial genomes exposed to different genotoxins and has proven to be particularly valuable in identifying reactive oxygen species-mediated mitochondrial DNA damage. QPCR can be used to quantify both the formation of DNA damage as well as the kinetics of damage removal. One of the main strengths of the assay is that it permits monitoring the integrity of mtDNA directly from total cellular DNA without the need for isolating mitochondria or a separate step of mitochondrial DNA purification. Here we discuss advantages and limitations of using QPCR to assay DNA damage in mammalian cells. In addition, we give a detailed protocol of the QPCR assay that helps facilitate its successful deployment in any molecular biology laboratory." @default.
- W55405400 created "2016-06-24" @default.
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- W55405400 creator A5015141183 @default.
- W55405400 creator A5028109597 @default.
- W55405400 creator A5055919829 @default.
- W55405400 date "2014-01-01" @default.
- W55405400 modified "2023-10-16" @default.
- W55405400 title "Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells" @default.
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- W55405400 doi "https://doi.org/10.1007/978-1-62703-739-6_31" @default.
- W55405400 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4407362" @default.
- W55405400 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24623245" @default.
- W55405400 hasPublicationYear "2014" @default.
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