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• W55663065 endingPage "546" @default.
• W55663065 startingPage "541" @default.
• W55663065 abstract "In Morocco, Leishmania infantum is the main etiologic agent of human and canine visceral leishmaniasis (VL). This species has been proven to be an opportunistic agent in HIV+ patients and is also responsible of sporadic cutaneous leishmaniasis (CL).This work aims to evaluate the genetic variability of Moroccan L. infantum strains based on PCR-RFLP analysis of the kinetoplastid DNA (kDNA) minicircles. A total of 75 DNA samples extracted from positive Giemsa-stained smears (n = 32) and from L. infantum cultures (n = 43) was studied. The samples have been taken from VL patients infected (n = 7) or not (n = 56) by HIV, patients with CL (n = 2) and finally from infected dogs (n = 10). An hypervariable region of kDNA was amplified using the primers MC1 and MC2; the PCR products were digested separately by a panel of nine restriction enzymes. The presence or absence of restriction fragments was scored in a binary matrix and the SplitsTree4 software was used for the construction of a Neighbor-Net network. Moroccan L. infantum population showed an important level of variability with the identification of 6 genotypes. For each genotype a PCR product was sequenced, confirming the presence of all the expected restriction sites. The predominant profile was the genotype B. A new genotype, named Q was detected for the first time, whereas the four other genotypes (G, K, N and O) were reported sporadically in the Mediterranean basin. The Neighbor-Net network segregates our L. infantum population into 3 clusters: Cluster I includes genotype B, cluster II grouping the genotypes O, Q and G and finally the cluster III contains the genotype N. The kDNA-PCR-RFLP assay is suitable for use directly on biological samples; it reveals an important degree of genetic variability among L. infantum strains even those belonging to the same zymodeme what is of great epidemiological interest." @default.
• W55663065 created "2016-06-24" @default.
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• W55663065 date "1996-09-01" @default.
• W55663065 modified "2023-09-23" @default.
• W55663065 title "AIDS and Leishmania infantum" @default.
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• W55663065 doi "https://doi.org/10.1016/0738-081x(96)00046-6" @default.
• W55663065 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8889333" @default.
• W55663065 hasPublicationYear "1996" @default.
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