Matches in SemOpenAlex for { <https://semopenalex.org/work/W56134116> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W56134116 endingPage "247" @default.
- W56134116 startingPage "233" @default.
- W56134116 abstract "Reporter gene fusions based on the enhanced green fluorescent protein (EGFP) are powerful experimental tools that allow real-time changes in gene expression to be monitored both in single cells and in populations. Here we describe the development of a chromosomally integrated transcriptional reporter fusion in Listeria monocytogenes that allows real-time measurements of gene expression. To construct a single copy of an EGFP-based fluorescent reporter fused to a promoter of interest (Px) in L. monocytogenes, a suicide shuttle vector carrying the Px::egfp gene fusion is first constructed in Escherichia coli (as an intermediate host). Then, the vector is transformed into L. monocytogenes and integrated into its chromosome by homologous recombination within the selected promoter region. Subsequently, analysis of fluorescence exhibited by cells carrying a single copy reporter can be performed under selected experimental conditions by stringent sample preparation, optimized image acquisition, and processing of the digital data with the image analysis freeware ImageJ. Thus, the methodology described here can be adapted to investigate the activity and regulation of any promoter in L. monocytogenes both at the cell and population levels." @default.
- W56134116 created "2016-06-24" @default.
- W56134116 creator A5036673095 @default.
- W56134116 creator A5055513243 @default.
- W56134116 date "2014-01-01" @default.
- W56134116 modified "2023-10-02" @default.
- W56134116 title "Using Enhanced Green Fluorescent Protein (EGFP) Promoter Fusions to Study Gene Regulation at Single Cell and Population Levels" @default.
- W56134116 cites W1804416415 @default.
- W56134116 cites W1968477104 @default.
- W56134116 cites W2078739770 @default.
- W56134116 cites W2079522174 @default.
- W56134116 cites W2099540110 @default.
- W56134116 cites W2133003941 @default.
- W56134116 cites W2149981735 @default.
- W56134116 cites W2166771618 @default.
- W56134116 doi "https://doi.org/10.1007/978-1-4939-0703-8_20" @default.
- W56134116 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24792563" @default.
- W56134116 hasPublicationYear "2014" @default.
- W56134116 type Work @default.
- W56134116 sameAs 56134116 @default.
- W56134116 citedByCount "6" @default.
- W56134116 countsByYear W561341162015 @default.
- W56134116 countsByYear W561341162016 @default.
- W56134116 countsByYear W561341162018 @default.
- W56134116 countsByYear W561341162020 @default.
- W56134116 countsByYear W561341162021 @default.
- W56134116 countsByYear W561341162022 @default.
- W56134116 crossrefType "book-chapter" @default.
- W56134116 hasAuthorship W56134116A5036673095 @default.
- W56134116 hasAuthorship W56134116A5055513243 @default.
- W56134116 hasConcept C101762097 @default.
- W56134116 hasConcept C102744134 @default.
- W56134116 hasConcept C104317684 @default.
- W56134116 hasConcept C111829193 @default.
- W56134116 hasConcept C123894998 @default.
- W56134116 hasConcept C142613039 @default.
- W56134116 hasConcept C144024400 @default.
- W56134116 hasConcept C145640855 @default.
- W56134116 hasConcept C149923435 @default.
- W56134116 hasConcept C150194340 @default.
- W56134116 hasConcept C153911025 @default.
- W56134116 hasConcept C161733203 @default.
- W56134116 hasConcept C173764188 @default.
- W56134116 hasConcept C2781350384 @default.
- W56134116 hasConcept C2908647359 @default.
- W56134116 hasConcept C33161422 @default.
- W56134116 hasConcept C40767141 @default.
- W56134116 hasConcept C523546767 @default.
- W56134116 hasConcept C54355233 @default.
- W56134116 hasConcept C86803240 @default.
- W56134116 hasConcept C92087593 @default.
- W56134116 hasConceptScore W56134116C101762097 @default.
- W56134116 hasConceptScore W56134116C102744134 @default.
- W56134116 hasConceptScore W56134116C104317684 @default.
- W56134116 hasConceptScore W56134116C111829193 @default.
- W56134116 hasConceptScore W56134116C123894998 @default.
- W56134116 hasConceptScore W56134116C142613039 @default.
- W56134116 hasConceptScore W56134116C144024400 @default.
- W56134116 hasConceptScore W56134116C145640855 @default.
- W56134116 hasConceptScore W56134116C149923435 @default.
- W56134116 hasConceptScore W56134116C150194340 @default.
- W56134116 hasConceptScore W56134116C153911025 @default.
- W56134116 hasConceptScore W56134116C161733203 @default.
- W56134116 hasConceptScore W56134116C173764188 @default.
- W56134116 hasConceptScore W56134116C2781350384 @default.
- W56134116 hasConceptScore W56134116C2908647359 @default.
- W56134116 hasConceptScore W56134116C33161422 @default.
- W56134116 hasConceptScore W56134116C40767141 @default.
- W56134116 hasConceptScore W56134116C523546767 @default.
- W56134116 hasConceptScore W56134116C54355233 @default.
- W56134116 hasConceptScore W56134116C86803240 @default.
- W56134116 hasConceptScore W56134116C92087593 @default.
- W56134116 hasLocation W561341161 @default.
- W56134116 hasLocation W561341162 @default.
- W56134116 hasOpenAccess W56134116 @default.
- W56134116 hasPrimaryLocation W561341161 @default.
- W56134116 hasRelatedWork W1989295391 @default.
- W56134116 hasRelatedWork W2021678023 @default.
- W56134116 hasRelatedWork W2053895538 @default.
- W56134116 hasRelatedWork W2354688522 @default.
- W56134116 hasRelatedWork W2363228671 @default.
- W56134116 hasRelatedWork W2368863504 @default.
- W56134116 hasRelatedWork W2373514221 @default.
- W56134116 hasRelatedWork W2378271209 @default.
- W56134116 hasRelatedWork W2389248981 @default.
- W56134116 hasRelatedWork W56134116 @default.
- W56134116 isParatext "false" @default.
- W56134116 isRetracted "false" @default.
- W56134116 magId "56134116" @default.
- W56134116 workType "book-chapter" @default.