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• W562127835 abstract "Decontamination of micro-organisms plays an important role in society. Applications range, for example, from the medical to food industries. Implementation of industrial sterilization systems reduces the likelihood of infection and cross-contamination. In the food industry, there has been an increasing incidence of microbial food poisoning over the last decade in Europe, Asia and the USA, with the result of significant loss of life. Medical applications are broad, and include for example sterilization of prosthesis, sterile packaging and clean room systems. There are, however, many approaches to achieve decontamination, and each process seems to have its niche application. Novel approaches have included: microwave, high pressure, pulsed electric fields and pulsed incoherent light. One of the more recent methods is the use of laser light. Lasers can be used in two ways to achieve decontamination, either by indirect use, where a photosensitize is applied to the surface, or directly, where the energy density delivered by the laser is sufficiently high to achieve killing. In the present case, the direct approach was used. Seven laser instruments, delivering radiation at a selection of wavelengths in the range of 0.355-118μm, were investigated for their ability to kill Escherichia coli as a lawned suspension of the bacteria on nutrient agar culture plates. One of the most effective was a 600 W CO2 laser operating at 10.6 μm, which produced 1.2 cm2 circular zones of sterilization at energy densities of around 8 Jcm−2 in a 30 ms exposure. The Nd:YAG laser was used to sterilize liquids inoculated with concentrations of E. coli ranging from 105 to 108 cells ml−1. The time-dependent heat diffusion equation was solved to calculate the three dimensional temperature distribution in the liquid and the death kinetics of laser inactivation were analysed.Decontamination of micro-organisms plays an important role in society. Applications range, for example, from the medical to food industries. Implementation of industrial sterilization systems reduces the likelihood of infection and cross-contamination. In the food industry, there has been an increasing incidence of microbial food poisoning over the last decade in Europe, Asia and the USA, with the result of significant loss of life. Medical applications are broad, and include for example sterilization of prosthesis, sterile packaging and clean room systems. There are, however, many approaches to achieve decontamination, and each process seems to have its niche application. Novel approaches have included: microwave, high pressure, pulsed electric fields and pulsed incoherent light. One of the more recent methods is the use of laser light. Lasers can be used in two ways to achieve decontamination, either by indirect use, where a photosensitize is applied to the surface, or directly, where the energy density..." @default.
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• W562127835 date "1997-01-01" @default.
• W562127835 modified "2023-09-27" @default.
• W562127835 title "Laser inactivation of E. coli" @default.
• W562127835 doi "https://doi.org/10.2351/1.5059756" @default.
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