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- W562276677 abstract "Scaffolded DNA origami enables the fabrication of a variety of complex nanostructures that promise utility in diverse fields of application, ranging from biosensing over advanced therapeutics to metamaterials. The broad applicability of DNA origami as a material beyond the level of proof-of-concept studies critically depends, among other factors, on the availability of large amounts of pure single-stranded scaffold DNA. Here, we present a method for the efficient production of M13 bacteriophage-derived genomic DNA using high-cell-density fermentation of Escherichia coli in stirred-tank bioreactors. We achieve phage titers of up to 1.6 × 1014 plaque-forming units per mL. Downstream processing yields up to 410 mg of high-quality single-stranded DNA per one liter reaction volume, thus upgrading DNA origami-based nanotechnology from the milligram to the gram scale." @default.
- W562276677 created "2016-06-24" @default.
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- W562276677 date "2015-06-03" @default.
- W562276677 modified "2023-10-17" @default.
- W562276677 title "Efficient Production of Single-Stranded Phage DNA as Scaffolds for DNA Origami" @default.
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- W562276677 doi "https://doi.org/10.1021/acs.nanolett.5b01461" @default.
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