FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W563270466> ?p ?o ?g. }

• W563270466 abstract "Diatoms are a divergent group of organisms with a complex evolutionary background, and belong to the Heterokonta group phylogenetically. Diatoms have gone through several endosymbiotic processes with both green algae and red algae, and have acquired a complex genome. Some of the genes and protein found in diatoms are similar to those found in plants and green algae, while others show similarities to animals (metazoa), yeast, bacteria and other unicellular microorganisms. This genomic diversity could be one of the reasons why diatoms dominate the primary production in many marine and fresh water ecosystems. Seminavis robusta is a benthic diatom that lives on the bottom sediments and has a bilateral symmetry. One of the advantages with S. robusta is the size; it is bigger than both Phaeodactylum tricornutum and Thalassiosira pseudonana which have been fully genome sequenced. S. robusta also have two mating types, which means that it is possible to do breeding between individuals and keep a pedigree. S. robusta has therefore been considered a good model organism for studies on benthic diatoms. In this study two experiments were done on S. robusta. The first experiment was to make a vector construct that could be used to transform S. robusta. The endogenous AtpBE/D promoter in S. robusta expressing the ATP synthase beta subunit and ATP synthase epsilon or delta (in this thesis referred to as epsilon) subunit was chosen as promoter for the construct. The AtpBE promoter was chosen because of its small size and because it is bidirectional and can express two genes simultaneously. The AtpBE promoter and the belonging AtpB and AtpE terminators were isolated from genomic DNA from S. robusta and cloned into the pBluescript (KS+) vector. The nourseothricin acetyltransferase (Nat1) gene was chosen as selectable maker. Nat1 gives resistance to nourseothricin, the gene was modified and synthetically synthesized to correspond to the tRNA abundance of S. robusta. The Nat1 gene was cloned into the expression of the AtpE direction of the promoter while Yellow Fluorescence Protein (YFP) was cloned into the AtpB side of the promoter. YFP was included to check the expression capability of the AtpB promoter direction. Results from transformation attempts showed that only Nat1 was expressed and translated in the cells. No YFP or YFP mRNA was detected, indicating that the gene was not transcribed. The second experiment performed on S. robusta was a light experiment, where cells in exponential growth phase were kept in dark for 12 hours before eight flasks were exposed to blue light (BL, 80 µmol photons m-2s-1), four flasks were set for 0,5 hour and the other four for 6 hours. After the exposure cells were harvested and RNA was isolated. The same procedure was done in white light (WL, 100 µmol photons m-2s-1) for 0,5 and 6 hours. The gene expressions from the treatments were studied with RT- qPCR and microarray technology. The study showed that some of the genes coding for Light Harvesting Complex proteins (LHCs), Aureochromes, Aureochrome-like proteins and Heat Shock Transcriptions factors (HSFs) had strong responses to the light treatments." @default.
• W563270466 created "2016-06-24" @default.
• W563270466 creator A5054734373 @default.
• W563270466 date "2014-01-01" @default.
• W563270466 modified "2023-09-24" @default.
• W563270466 title "Studies of light responses and the development of a transformation system for the benthic diatom Seminavis robusta" @default.
• W563270466 hasPublicationYear "2014" @default.
• W563270466 type Work @default.
• W563270466 sameAs 563270466 @default.
• W563270466 citedByCount "0" @default.
• W563270466 crossrefType "dissertation" @default.
• W563270466 hasAuthorship W563270466A5054734373 @default.
• W563270466 hasConcept C104317684 @default.
• W563270466 hasConcept C141231307 @default.
• W563270466 hasConcept C142796444 @default.
• W563270466 hasConcept C18903297 @default.
• W563270466 hasConcept C2777082925 @default.
• W563270466 hasConcept C2778902744 @default.
• W563270466 hasConcept C2780655307 @default.
• W563270466 hasConcept C2780838361 @default.
• W563270466 hasConcept C2780892065 @default.
• W563270466 hasConcept C54355233 @default.
• W563270466 hasConcept C559758991 @default.
• W563270466 hasConcept C59822182 @default.
• W563270466 hasConcept C83042747 @default.
• W563270466 hasConcept C86803240 @default.
• W563270466 hasConceptScore W563270466C104317684 @default.
• W563270466 hasConceptScore W563270466C141231307 @default.
• W563270466 hasConceptScore W563270466C142796444 @default.
• W563270466 hasConceptScore W563270466C18903297 @default.
• W563270466 hasConceptScore W563270466C2777082925 @default.
• W563270466 hasConceptScore W563270466C2778902744 @default.
• W563270466 hasConceptScore W563270466C2780655307 @default.
• W563270466 hasConceptScore W563270466C2780838361 @default.
• W563270466 hasConceptScore W563270466C2780892065 @default.
• W563270466 hasConceptScore W563270466C54355233 @default.
• W563270466 hasConceptScore W563270466C559758991 @default.
• W563270466 hasConceptScore W563270466C59822182 @default.
• W563270466 hasConceptScore W563270466C83042747 @default.
• W563270466 hasConceptScore W563270466C86803240 @default.
• W563270466 hasLocation W5632704661 @default.
• W563270466 hasOpenAccess W563270466 @default.
• W563270466 hasPrimaryLocation W5632704661 @default.
• W563270466 hasRelatedWork W1991459823 @default.
• W563270466 hasRelatedWork W2014486057 @default.
• W563270466 hasRelatedWork W2017151507 @default.
• W563270466 hasRelatedWork W2017389797 @default.
• W563270466 hasRelatedWork W2017820167 @default.
• W563270466 hasRelatedWork W2020953659 @default.
• W563270466 hasRelatedWork W2027042586 @default.
• W563270466 hasRelatedWork W2038303507 @default.
• W563270466 hasRelatedWork W2043343933 @default.
• W563270466 hasRelatedWork W2050081728 @default.
• W563270466 hasRelatedWork W2055923801 @default.
• W563270466 hasRelatedWork W2063884336 @default.
• W563270466 hasRelatedWork W2071032540 @default.
• W563270466 hasRelatedWork W2079596879 @default.
• W563270466 hasRelatedWork W2090068027 @default.
• W563270466 hasRelatedWork W2109759564 @default.
• W563270466 hasRelatedWork W2315780093 @default.
• W563270466 hasRelatedWork W2381484725 @default.
• W563270466 hasRelatedWork W2383194606 @default.
• W563270466 hasRelatedWork W3023696406 @default.
• W563270466 isParatext "false" @default.
• W563270466 isRetracted "false" @default.
• W563270466 magId "563270466" @default.
• W563270466 workType "dissertation" @default.