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- W563540271 abstract "The formation of singlet molecular oxygen (1O2) has been shown to occur during photoexcitation of a variety of biological and also clinically applied compounds, such as porphyrins, psoralens and tetracyclines (see, for example references 1–3), but also occurs during enzymatic reactions including cyclooxygenase4 and chloroperoxidase.5 Regarding the biological consequences of 1O2 formation, DNA is of particular interest. 1O2 is known to react with guanine 6,7; however, results on biologically active DNA were conflicting, in particular regarding 1O2-dependent strand-break formation under neutral conditions, i.e. without alkaline treatment. 8-11 Here we emphasize two aspects of 1O2-mediated damage. First, the effect of 1O2 on the transforming activity of pBR322 and M1 3 DNA in E. coli is presented. Second, two different sources of 1O2 are employed for investigation of strand-break formation; these are microwave discharge and the thermodissociable endoperoxide of disodium 3,3′-(1,4-naphthylidene)dipropionate (NDPO2)." @default.
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- W563540271 date "1988-01-01" @default.
- W563540271 modified "2023-09-27" @default.
- W563540271 title "Effects of Singlet Oxygen on the Biological Activity of DNA and Its Involvement in Single Strand-Break Formation" @default.
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- W563540271 doi "https://doi.org/10.1007/978-1-4684-5568-7_73" @default.
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