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- W56407785 abstract "Fermentation conditions for the production of lipase by Bacillus stearothermophilus S-203 have been established in 300 ml baffled shake flasks. The optimum medium consists of yeast extract 0.3%, ammonium acetate 0.5%, cotton-seed soil 0.4%, sucrose 0.5%, Tween 40 0.4%, phosphate buffer 0.04 M pH 6.9, and other inorganic salts. The optimum temperature is 45–55 °C, volume per flask 50 ml, shaking speed 300 rpm, and incubation time 24 hours. Under these conditions the yield of lipase was 59.5 units per ml. Es wurden die Fermentationsbedingungen zur Erzeugung von Lipase durch Bacillus stearothermophilus ermittelt. Das am besten geeignete Medium besteht aus Hefeextrakt (0.3 %), Ammoniumazetat (0,5%), Baumwollsamenöl (0,4%), Rohrzucker (0,5%), Tween 40 (0,4%), Phosphatpuffer (0,04 M, pH 6,9) und weiteren anorganischen Salzen. Die optimale Temperatur ist 45–55 °C, der Flascheninhalt 50 ml, die Schüttelgeschwindigkeit 300 U/Min. und die Inkubationszeit 24 Stunden. Unter diesen Bedingungen wurden 59,5 Einheiten Lipase pro ml gebildet." @default.
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- W56407785 date "1984-01-01" @default.
- W56407785 modified "2023-10-14" @default.
- W56407785 title "Lipase production by Bacillus stearothermophilus S-203 in shake flasks" @default.
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- W56407785 doi "https://doi.org/10.1016/s0232-4393(84)80034-7" @default.
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