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- W564368932 abstract "The specific primers P29A/P29B and PEANT1/PEANT2 derived from plasmid pEA29 of Erwinia amylovora were selected to combine nested PCR method for the detection of trace E.amylovora in imported apple fruits with a limit as low as 0.15 pg of total DNA.From 166 samples of imported apple fruits from USA,Japan,New Zealand and Chile,this detection method achieved better results than nested PCR in a single closed tube that EPPO recommended and standard PCR with 53.6%,38.0% and 8.4% of positive samples,respectively.This nested PCR method was proposed to be used for the detection of E.amylovora in imported commercial apple fruits.The results of detection verified the possibility of imported commercial apple fruits harbouring E.amylovora in the calyxes and fruit stalks." @default.
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- W564368932 date "2009-01-01" @default.
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- W564368932 title "Detection of Erwinia amylovora in imported apple fruit with nested PCR." @default.
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