Matches in SemOpenAlex for { <https://semopenalex.org/work/W56534538> ?p ?o ?g. }
- W56534538 endingPage "4927" @default.
- W56534538 startingPage "4919" @default.
- W56534538 abstract "Vascular endothelial growth factor (VEGF) is an angiogenic inducer that mediates its effects through two high affinity receptor tyrosine kinases, Flt-1 and KDR. Flt-1 is required for endothelial cell morphogenesis whereas KDR is involved primarily in mitogenesis. Flt-1 has an alternative ligand, placenta growth factor (PlGF). Both Flt-1 and KDR have seven immunoglobulin (Ig)-like domains in the extracellular domain. The significance and function of these domains for ligand binding and receptor activation are unknown. Here we show that deletion of the second domain of Flt-1 completely abolishes the binding of VEGF. Introduction of the second domain of KDR into an Flt-1 mutant lacking the homologous domain restored VEGF binding. However, the ligand specificity was characteristic of the KDR receptor. We then created chimeric receptors where the first three or just the second Ig-like domains of Flt-1 replaced the corresponding domains in Flt-4, a receptor that does not bind VEGF, and analyzed their ability to bind VEGF. Both swaps conferred upon Flt-4 the ability to bind VEGF with an affinity nearly identical to that of wild-type Flt-1. Furthermore, transfected cells expressing these chimeric Flt-4 receptors exhibited increased DNA synthesis in response to VEGF or PlGF. These results demonstrate that a single Ig-like domain is the major determinant for VEGF-PlGF interaction and that binding to this domain may initiate a signal transduction cascade." @default.
- W56534538 created "2016-06-24" @default.
- W56534538 creator A5013356370 @default.
- W56534538 creator A5037270460 @default.
- W56534538 creator A5048350728 @default.
- W56534538 creator A5049237460 @default.
- W56534538 creator A5088935340 @default.
- W56534538 date "1996-09-01" @default.
- W56534538 modified "2023-09-27" @default.
- W56534538 title "The second immunoglobulin-like domain of the VEGF tyrosine kinase receptor Flt-1 determines ligand binding and may initiate a signal transduction cascade." @default.
- W56534538 cites W10096759 @default.
- W56534538 cites W1489873347 @default.
- W56534538 cites W1532679424 @default.
- W56534538 cites W1537152740 @default.
- W56534538 cites W1568057583 @default.
- W56534538 cites W1587020653 @default.
- W56534538 cites W1600542667 @default.
- W56534538 cites W172183759 @default.
- W56534538 cites W1741848512 @default.
- W56534538 cites W1875150791 @default.
- W56534538 cites W1969328947 @default.
- W56534538 cites W1977158361 @default.
- W56534538 cites W1981612052 @default.
- W56534538 cites W1986559037 @default.
- W56534538 cites W1987644022 @default.
- W56534538 cites W2000000541 @default.
- W56534538 cites W2003598961 @default.
- W56534538 cites W2008940139 @default.
- W56534538 cites W2023453327 @default.
- W56534538 cites W2028825060 @default.
- W56534538 cites W2029763523 @default.
- W56534538 cites W2031209863 @default.
- W56534538 cites W2032359283 @default.
- W56534538 cites W2034220289 @default.
- W56534538 cites W2036931150 @default.
- W56534538 cites W2038039729 @default.
- W56534538 cites W2043850275 @default.
- W56534538 cites W2045247659 @default.
- W56534538 cites W2046516822 @default.
- W56534538 cites W2049569341 @default.
- W56534538 cites W2054154416 @default.
- W56534538 cites W20584199 @default.
- W56534538 cites W2059436482 @default.
- W56534538 cites W2066043796 @default.
- W56534538 cites W2069092801 @default.
- W56534538 cites W2074969682 @default.
- W56534538 cites W2075086348 @default.
- W56534538 cites W2084506573 @default.
- W56534538 cites W2093498435 @default.
- W56534538 cites W2103174300 @default.
- W56534538 cites W2121220148 @default.
- W56534538 cites W2147274651 @default.
- W56534538 cites W2162249561 @default.
- W56534538 cites W2166156136 @default.
- W56534538 cites W2175146729 @default.
- W56534538 cites W2324588265 @default.
- W56534538 cites W2340295567 @default.
- W56534538 cites W2413477926 @default.
- W56534538 cites W4290247175 @default.
- W56534538 cites W92307352 @default.
- W56534538 doi "https://doi.org/10.1002/j.1460-2075.1996.tb00872.x" @default.
- W56534538 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/452229" @default.
- W56534538 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8890165" @default.
- W56534538 hasPublicationYear "1996" @default.
- W56534538 type Work @default.
- W56534538 sameAs 56534538 @default.
- W56534538 citedByCount "191" @default.
- W56534538 countsByYear W565345382012 @default.
- W56534538 countsByYear W565345382013 @default.
- W56534538 countsByYear W565345382014 @default.
- W56534538 countsByYear W565345382015 @default.
- W56534538 countsByYear W565345382016 @default.
- W56534538 countsByYear W565345382017 @default.
- W56534538 countsByYear W565345382018 @default.
- W56534538 countsByYear W565345382019 @default.
- W56534538 countsByYear W565345382020 @default.
- W56534538 countsByYear W565345382021 @default.
- W56534538 countsByYear W565345382022 @default.
- W56534538 countsByYear W565345382023 @default.
- W56534538 crossrefType "journal-article" @default.
- W56534538 hasAuthorship W56534538A5013356370 @default.
- W56534538 hasAuthorship W56534538A5037270460 @default.
- W56534538 hasAuthorship W56534538A5048350728 @default.
- W56534538 hasAuthorship W56534538A5049237460 @default.
- W56534538 hasAuthorship W56534538A5088935340 @default.
- W56534538 hasBestOaLocation W565345382 @default.
- W56534538 hasConcept C101544691 @default.
- W56534538 hasConcept C146285616 @default.
- W56534538 hasConcept C14858245 @default.
- W56534538 hasConcept C153911025 @default.
- W56534538 hasConcept C167734588 @default.
- W56534538 hasConcept C170493617 @default.
- W56534538 hasConcept C177917778 @default.
- W56534538 hasConcept C2777025900 @default.
- W56534538 hasConcept C42362537 @default.
- W56534538 hasConcept C502942594 @default.
- W56534538 hasConcept C55493867 @default.
- W56534538 hasConcept C62478195 @default.