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- W56576371 abstract "The purpose of this study is to determine the expression of the MRL class retroviral elements that may undergo frequent retrotransposition in the mouse germline, as suggested by the previous studies of this laboratory. A quantity of 300,000 plaque forming units of recombinant lambda phage containing mouse (Mus musculus) testes cDNA population were used to infect E. coli C 600 Hfl and subsequently were blotted onto nitrocellulose filters. Numbers of recombinant phage plaques containing retroviral genomic sequences were determined by hybridization of the nitrocellulose membrane filters with specific molecular probes representing the MRL elements and murine leukemia virus-related proviruses. From analysis of autoradiographs the observed mean numbers of positive clones per 10,000 plaque forming units were as determined. The results indicate relatively high content of MRL element transcripts in the mouse testis. Whether selective members or all the MRL multiple gene family in the mouse genome are involved in the expression remains to be investigated." @default.
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- W56576371 date "1989-04-01" @default.
- W56576371 modified "2023-09-24" @default.
- W56576371 title "Determination of the frequency of several retroviral genomic elements in the cDNA from mouse testes" @default.
- W56576371 hasPublicationYear "1989" @default.
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