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- W565970276 abstract "プロテアーゼのリフォールディングにおける主な妨害反応である自己消化を抑制する日的で合成インヒビターを活用した．通常の希釈法では15%程度の活性回復率しか得られないStreptomycesgriseus由来のトリプシン（SGT）のりフォールディング系に，インヒビターであるp一アミノベンザミジンを固定化したゲルを共存させ，活性回復率を60%を越えるまでに上昇させ，再生したSGTの精製・回収を効率よく達成できた.さらに，この懸濁系を用いてもう一つの主な妨害反応である凝集反応を，1Mureaの共存により顕著に減少させ，8O%強の回復率を得ることができた-また，インヒビター固定化ゲルが再使用可能なことから，連続的なリフォールディング操作に移行できることが明らかとなった．" @default.
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- W565970276 date "2001-01-01" @default.
- W565970276 modified "2023-09-24" @default.
- W565970276 title "Bioseparation Engineering. Achievement of High Recovered Activity and Efficient Purification and Recovery by Application of Immobilized Inhibitor to Protease Refolding." @default.
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- W565970276 doi "https://doi.org/10.1252/kakoronbunshu.27.169" @default.
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