Matches in SemOpenAlex for { <https://semopenalex.org/work/W568420834> ?p ?o ?g. }
- W568420834 abstract "The central dogma of biology asserts that DNA is transcribed into RNA and RNA is translated into protein. However, this overtly simplistic assertion fails to portray the highly orchestrated and regulated mechanisms of transcription and translation. During the process of transcription, RNA provides the template for translation and protein synthesis as well as the structural and sequence specificity of many RNA and protein-based machines. While only 1-5% of the genome will escape the nucleus to be translated as mRNAs, complex, parallel, highly-conserved mechanisms have evolved to regulate specific mRNAs. Trans-acting factors bind cis-elements in both the 5 and 3 untranslated regions of mRNA to regulate their stability, localization, and translation. While a few salient examples have been elucidated over the last few decades, mRNA translation can be reversibly regulated by the shortening and lengthening of the 3 polyadenylate tail of mRNA. CPEB, an important factor that nucleates a complex of proteins to regulate the polyadenylate tail of mRNA, exemplifies a major paradigm of translational control during oocyte maturation and early development. CPEB function is also conserved in neurons and somatic foreskin fibroblasts where it plays an important role in protein synthesis dependent synaptic plasticity and senescence respectively. Focusing on the function of CPEB and its role in mRNA polyadenylation during human cellular senescence, the following dissertation documents the important finding that CPEB is required for the normal polyadenylation of p53 mRNA necessary for its normal translation and onset of senescence. Cells that lack CPEB have abnormal levels of mitochondria and ROS production, which are demonstrated to arise from the direct result of hypomorphic p53 levels. Finally, in an attempt to recapitulate the model of CPEB complex polyadenylation in human somatic cells, I unexpectedly find that Gld-2, a poly(A) polymerase required for CPEB-mediated polyadenylation in Xenopus laevis oocytes, is not required for p53 polyadenylation, but instead regulates the stability of a microRNA that in turn regulates CPEB mRNA translation. Furthermore, I demonstrate that CPEB requires Gld-4 for the normal polyadenylation and translation of p53 mRNA." @default.
- W568420834 created "2016-06-24" @default.
- W568420834 creator A5074463664 @default.
- W568420834 date "2010-01-01" @default.
- W568420834 modified "2023-09-23" @default.
- W568420834 title "Post-Transcriptional Control of Human Cellular Senescence: A Dissertation" @default.
- W568420834 cites W1486592771 @default.
- W568420834 cites W1488368159 @default.
- W568420834 cites W1517051009 @default.
- W568420834 cites W1553081734 @default.
- W568420834 cites W1554943836 @default.
- W568420834 cites W1565524475 @default.
- W568420834 cites W1591026223 @default.
- W568420834 cites W1625157788 @default.
- W568420834 cites W177280810 @default.
- W568420834 cites W1784570168 @default.
- W568420834 cites W1908439727 @default.
- W568420834 cites W1964177189 @default.
- W568420834 cites W1965149500 @default.
- W568420834 cites W1968220278 @default.
- W568420834 cites W1969104747 @default.
- W568420834 cites W1970924670 @default.
- W568420834 cites W1972979813 @default.
- W568420834 cites W1973433187 @default.
- W568420834 cites W1973480874 @default.
- W568420834 cites W1974676736 @default.
- W568420834 cites W1980084095 @default.
- W568420834 cites W1980878904 @default.
- W568420834 cites W1982163838 @default.
- W568420834 cites W1982395176 @default.
- W568420834 cites W1983651061 @default.
- W568420834 cites W1984397453 @default.
- W568420834 cites W1984721390 @default.
- W568420834 cites W1985986952 @default.
- W568420834 cites W1986143744 @default.
- W568420834 cites W1986699200 @default.
- W568420834 cites W1987777270 @default.
- W568420834 cites W1992935306 @default.
- W568420834 cites W1993959671 @default.
- W568420834 cites W1993995783 @default.
- W568420834 cites W1994142477 @default.
- W568420834 cites W1996394440 @default.
- W568420834 cites W1998594964 @default.
- W568420834 cites W2002840452 @default.
- W568420834 cites W2005670411 @default.
- W568420834 cites W2006129738 @default.
- W568420834 cites W2006563243 @default.
- W568420834 cites W2006612594 @default.
- W568420834 cites W2008410413 @default.
- W568420834 cites W2010081999 @default.
- W568420834 cites W2013756663 @default.
- W568420834 cites W2018116689 @default.
- W568420834 cites W2019167811 @default.
- W568420834 cites W2020330574 @default.
- W568420834 cites W2021484263 @default.
- W568420834 cites W2022553267 @default.
- W568420834 cites W2025304958 @default.
- W568420834 cites W2026051181 @default.
- W568420834 cites W2027779909 @default.
- W568420834 cites W2028845751 @default.
- W568420834 cites W2028933307 @default.
- W568420834 cites W2030963645 @default.
- W568420834 cites W2032585944 @default.
- W568420834 cites W2036379239 @default.
- W568420834 cites W2036676765 @default.
- W568420834 cites W2037437212 @default.
- W568420834 cites W2037528259 @default.
- W568420834 cites W2037786477 @default.
- W568420834 cites W2041037918 @default.
- W568420834 cites W2044025778 @default.
- W568420834 cites W2044273755 @default.
- W568420834 cites W2045440227 @default.
- W568420834 cites W2045462467 @default.
- W568420834 cites W2046524941 @default.
- W568420834 cites W2046620209 @default.
- W568420834 cites W2047733032 @default.
- W568420834 cites W2048146797 @default.
- W568420834 cites W2050002679 @default.
- W568420834 cites W2051127482 @default.
- W568420834 cites W2052480299 @default.
- W568420834 cites W205399872 @default.
- W568420834 cites W2056172652 @default.
- W568420834 cites W2057549495 @default.
- W568420834 cites W2057639603 @default.
- W568420834 cites W2057728173 @default.
- W568420834 cites W2058220225 @default.
- W568420834 cites W2061619522 @default.
- W568420834 cites W2061784180 @default.
- W568420834 cites W2062515127 @default.
- W568420834 cites W2065677302 @default.
- W568420834 cites W2066741869 @default.
- W568420834 cites W2067064841 @default.
- W568420834 cites W2067117191 @default.
- W568420834 cites W2069930342 @default.
- W568420834 cites W2070739894 @default.
- W568420834 cites W2072569792 @default.
- W568420834 cites W2072636314 @default.
- W568420834 cites W2073738336 @default.
- W568420834 cites W2076658638 @default.
- W568420834 cites W2076668993 @default.