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- W568740863 abstract "Bu çalışmada H120 suşu ile aşılanmış 2 broyler ve 8 damızlık sürüde infeksiyöz bronşit şüpheli problemlere rastlanmış 2 sürüye ait 60 broyler tavuktan (30 svap/sürü) ve 8 sürüye ait 160 damızlık tavuktan (20 svap/sürü) özellikle solunum bulguları ile nefrit lezyonları gösterenlerden toplam 220 trakeal svap infeksiyöz bronşit virusu (IBV)’nu reverse transcriptase polymerase chain reaction (RT-PCR) ile belirlemek ve IBV’yi izole etmek amacı ile alındı. IBV’nin trakeal svap örneklerinden direkt olarak RT-PCR ile deteksiyonu embryolu yumurtaların allantoik sıvılarından IBV izolasyonu ve bu örneklerden RT-PCR yapılması ile doğrulandı. Basic Local Alignment Search Tool (BLAST) sonuçları 8 Türk izolatının EU780077 (IS/1494/06 IBV suşu) ile %99 benzerlik gösterdiğini ortaya koydu. Broyler sürüden TR_IS1494_06_B12011 ve damızlık sürüden TR_1494_06_7201 olarak seçilen temsili iki izolatın dizilerinin filogenetik ve sıralama analizleri de IS/1494/06 IBV genotipine yakınlığı doğruladı. Sonuç olarak, Türkiye’de IBV H120 suşu ile aşılamaya rağmen IB’den korunamayan tavuklardan ilk defa izole edilmesi ve IS/1494/06 olarak genotiplendirilmesi bu IBV’nin Orta Doğu ülkelerinden taşınma olasılığını akla getirmektedir" @default.
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- W568740863 date "2013-01-01" @default.
- W568740863 modified "2023-10-14" @default.
- W568740863 title "Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey" @default.
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- W568740863 doi "https://doi.org/10.1501/vetfak_0000002549" @default.
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