Matches in SemOpenAlex for { <https://semopenalex.org/work/W568766616> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W568766616 endingPage "12" @default.
- W568766616 startingPage "7" @default.
- W568766616 abstract "【Objective】 The study was to investigate the influence of peptide SIINFEKL concentration and Dendritic Cell(DC) number on primary CD8+ T cell differentiation and proliferation.【Method】 Bone marrow-derived DCs were generated from bone marrow cells in the presence of GM-CSF and IL-4.OT-I mice CD8+ T cells were isolated by anti-mCD8 conjugated magnetic microbeads,and stimulated by SIINFEKL peptides and mature dendritic cells(maDC).Serial diluted peptides SIINFEKL(100,10,1,0.1,0.01,0.001 ng/mL) and different amounts of DCs(DC/T ratio of 1/20,1/5) were co-cultured with CD8+ T cells to stimulate the activation of CD8+ T cells.Then the cell number,division rate and activated surface marker of co-cultured CD8+ T cells were analyzed by flow cytometry,and cell vitality was measured by propidium iodide(PI) stain.【Result】 Deficient peptides result in insufficient CD8+ T cell proliferation,while excess peptides impair live CD8+ T cell number.CD8+ T cell proliferation was more pronounced when more DCs were used to present peptides ranging from 0.001 ng/mL to 0.1 ng/mL and fewer DCs were used to present peptides ranging from 1 ng/mL to 100 ng/mL.High concentration of peptide antigen or large numbers of DCs lead to the fully activation and division of CD8+ T cells but followed a remarkable decrease in CD8+ T cell number,which may be caused by activation induced cell death(AICD).【Conclusion】 Effective CD8+ T cell activation and proliferation depend on the optimal intensity of antigenic signals,which is controlled by the amount of both peptide antigen and DCs.To induce effective and prolonged cellular immune responses,the antigenic stimulation should meet the minimum requirement of activation,and elaborately evaluate to avoid AICD." @default.
- W568766616 created "2016-06-24" @default.
- W568766616 creator A5016383187 @default.
- W568766616 creator A5018299241 @default.
- W568766616 creator A5049893641 @default.
- W568766616 creator A5063982212 @default.
- W568766616 creator A5076004121 @default.
- W568766616 creator A5083247969 @default.
- W568766616 date "2009-01-01" @default.
- W568766616 modified "2023-09-26" @default.
- W568766616 title "Influence of antigen dose and DC number on CD8+ T cell differentiation and proliferation of OT-I transgenic mice." @default.
- W568766616 hasPublicationYear "2009" @default.
- W568766616 type Work @default.
- W568766616 sameAs 568766616 @default.
- W568766616 citedByCount "0" @default.
- W568766616 crossrefType "journal-article" @default.
- W568766616 hasAuthorship W568766616A5016383187 @default.
- W568766616 hasAuthorship W568766616A5018299241 @default.
- W568766616 hasAuthorship W568766616A5049893641 @default.
- W568766616 hasAuthorship W568766616A5063982212 @default.
- W568766616 hasAuthorship W568766616A5076004121 @default.
- W568766616 hasAuthorship W568766616A5083247969 @default.
- W568766616 hasConcept C147483822 @default.
- W568766616 hasConcept C153911025 @default.
- W568766616 hasConcept C154317977 @default.
- W568766616 hasConcept C167672396 @default.
- W568766616 hasConcept C185592680 @default.
- W568766616 hasConcept C202751555 @default.
- W568766616 hasConcept C203014093 @default.
- W568766616 hasConcept C2776090121 @default.
- W568766616 hasConcept C2778170410 @default.
- W568766616 hasConcept C553184892 @default.
- W568766616 hasConcept C55493867 @default.
- W568766616 hasConcept C62112901 @default.
- W568766616 hasConcept C86803240 @default.
- W568766616 hasConcept C8891405 @default.
- W568766616 hasConcept C95444343 @default.
- W568766616 hasConceptScore W568766616C147483822 @default.
- W568766616 hasConceptScore W568766616C153911025 @default.
- W568766616 hasConceptScore W568766616C154317977 @default.
- W568766616 hasConceptScore W568766616C167672396 @default.
- W568766616 hasConceptScore W568766616C185592680 @default.
- W568766616 hasConceptScore W568766616C202751555 @default.
- W568766616 hasConceptScore W568766616C203014093 @default.
- W568766616 hasConceptScore W568766616C2776090121 @default.
- W568766616 hasConceptScore W568766616C2778170410 @default.
- W568766616 hasConceptScore W568766616C553184892 @default.
- W568766616 hasConceptScore W568766616C55493867 @default.
- W568766616 hasConceptScore W568766616C62112901 @default.
- W568766616 hasConceptScore W568766616C86803240 @default.
- W568766616 hasConceptScore W568766616C8891405 @default.
- W568766616 hasConceptScore W568766616C95444343 @default.
- W568766616 hasIssue "9" @default.
- W568766616 hasLocation W5687666161 @default.
- W568766616 hasOpenAccess W568766616 @default.
- W568766616 hasPrimaryLocation W5687666161 @default.
- W568766616 hasRelatedWork W1556853316 @default.
- W568766616 hasRelatedWork W16608652 @default.
- W568766616 hasRelatedWork W1889902259 @default.
- W568766616 hasRelatedWork W1967467757 @default.
- W568766616 hasRelatedWork W1970610994 @default.
- W568766616 hasRelatedWork W1976860874 @default.
- W568766616 hasRelatedWork W2034262749 @default.
- W568766616 hasRelatedWork W2065469185 @default.
- W568766616 hasRelatedWork W2071003322 @default.
- W568766616 hasRelatedWork W2096305603 @default.
- W568766616 hasRelatedWork W2138573063 @default.
- W568766616 hasRelatedWork W2372163874 @default.
- W568766616 hasRelatedWork W2384682791 @default.
- W568766616 hasRelatedWork W2416606236 @default.
- W568766616 hasRelatedWork W2548031675 @default.
- W568766616 hasRelatedWork W2555732681 @default.
- W568766616 hasRelatedWork W2562253532 @default.
- W568766616 hasRelatedWork W2576549454 @default.
- W568766616 hasRelatedWork W3030374359 @default.
- W568766616 hasRelatedWork W3186578719 @default.
- W568766616 hasVolume "37" @default.
- W568766616 isParatext "false" @default.
- W568766616 isRetracted "false" @default.
- W568766616 magId "568766616" @default.
- W568766616 workType "article" @default.