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- W569257380 abstract "Objective To establish a group-specific Terminal Restriction Fragment Length Polymorphisms(T-RFLP) method to analyze the structure of Clostridium leptum group in human gut samples collected from large-scale populations.Method The reproducibility and sensitivity of Clostridium leptum group specific T-RFLP was determined.T-RFLP and DGGE were used to analyze the fecal Clostridium leptum subgroup of 15 human subjects and the results were compared to evaluate the consistency of the two techniques.Result The Clostridium leptum group-specific T-RFLP method was shown to be of high reproducibility and had the detection limit down to 1%.The result of T-RFLP was in good coherence with that of DGGE.Conclusion This group-specific T-RFLP method could be used to efficiently screen and compare the structure of Clostridium leptum group in large quantity of fecal samples." @default.
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- W569257380 date "2009-01-01" @default.
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- W569257380 title "The application of T-RFLP in analyzing Clostridium leptum group in human gut microbiota." @default.
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