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- W570018495 abstract "Rab is a small GTP-binding protein family that regulates various pathways of vesicular transport. Although more than 60 Rab proteins are targeted to specific organelles in mammalian cells, the mechanisms underlying the specificity of Rab proteins for the respective organelles remain unknown. In this study, we reconstituted the Golgi targeting of Rab6A in streptolysin O (SLO)-permeabilized HeLa cells in a cytosol-dependent manner and investigated the biochemical requirements of targeting. Golgi-targeting assays identified Bicaudal-D (BICD)2, which is reportedly involved in the dynein-mediated transport of mRNAs during oogenesis and embryogenesis in Drosophila, as a cytosolic factor for the Golgi targeting of Rab6A in SLO-permeabilized HeLa cells. Subsequent immunofluorescence analyses indicated decreased amounts of the GTP-bound active form of Rab6 in BICD2-knockdown cells. In addition, fluorescence recovery after photobleaching (FRAP) analyses revealed that overexpression of the C-terminal region of BICD2 decreased the exchange rate of GFP-Rab6A between the Golgi membrane and the cytosol. Collectively, these results indicated that BICD2 facilitates the binding of Rab6A to the Golgi by stabilizing its GTP-bound form. Moreover, several analyses of vesicular transport demonstrated that Rab6A and BICD2 play crucial roles in Golgi tubule fusion with the endoplasmic reticulum (ER) in brefeldin A (BFA)-treated cells, indicating that BICD2 is involved in coat protein I (COPI)-independent Golgi-to-ER retrograde vesicular transport." @default.
- W570018495 created "2016-06-24" @default.
- W570018495 creator A5034921892 @default.
- W570018495 creator A5048937040 @default.
- W570018495 creator A5059084077 @default.
- W570018495 date "2015-10-01" @default.
- W570018495 modified "2023-10-14" @default.
- W570018495 title "Reconstitution of the targeting of Rab6A to the Golgi apparatus in semi-intact HeLa cells: A role of BICD2 in stabilizing Rab6A on Golgi membranes and a concerted role of Rab6A/BICD2 interactions in Golgi-to-ER retrograde transport" @default.
- W570018495 cites W1519120665 @default.
- W570018495 cites W1541290836 @default.
- W570018495 cites W1565184249 @default.
- W570018495 cites W1579960871 @default.
- W570018495 cites W1595083713 @default.
- W570018495 cites W1606305740 @default.
- W570018495 cites W1936909041 @default.
- W570018495 cites W1968774879 @default.
- W570018495 cites W1970037495 @default.
- W570018495 cites W1970166276 @default.
- W570018495 cites W1970654084 @default.
- W570018495 cites W1979099792 @default.
- W570018495 cites W1980906848 @default.
- W570018495 cites W1987564626 @default.
- W570018495 cites W1993179214 @default.
- W570018495 cites W1994668150 @default.
- W570018495 cites W1996281893 @default.
- W570018495 cites W1999432764 @default.
- W570018495 cites W1999786030 @default.
- W570018495 cites W2000226738 @default.
- W570018495 cites W2000686839 @default.
- W570018495 cites W2011104938 @default.
- W570018495 cites W2011593319 @default.
- W570018495 cites W2014382524 @default.
- W570018495 cites W2016051706 @default.
- W570018495 cites W2026283030 @default.
- W570018495 cites W2030939970 @default.
- W570018495 cites W2031053226 @default.
- W570018495 cites W2034403065 @default.
- W570018495 cites W2037135039 @default.
- W570018495 cites W2038570204 @default.
- W570018495 cites W2038966235 @default.
- W570018495 cites W2040897146 @default.
- W570018495 cites W2047730219 @default.
- W570018495 cites W2049620445 @default.
- W570018495 cites W2050213416 @default.
- W570018495 cites W2055041189 @default.
- W570018495 cites W2055374398 @default.
- W570018495 cites W2056003065 @default.
- W570018495 cites W2061867367 @default.
- W570018495 cites W2062793212 @default.
- W570018495 cites W2065635914 @default.
- W570018495 cites W2066815088 @default.
- W570018495 cites W2069039144 @default.
- W570018495 cites W2071534681 @default.
- W570018495 cites W2088500487 @default.
- W570018495 cites W2091205354 @default.
- W570018495 cites W2092360229 @default.
- W570018495 cites W2094104389 @default.
- W570018495 cites W2095554275 @default.
- W570018495 cites W2099396320 @default.
- W570018495 cites W2100096634 @default.
- W570018495 cites W2101207629 @default.
- W570018495 cites W2104572704 @default.
- W570018495 cites W2110817313 @default.
- W570018495 cites W2112078762 @default.
- W570018495 cites W2114139789 @default.
- W570018495 cites W2117398553 @default.
- W570018495 cites W2124421509 @default.
- W570018495 cites W2129463316 @default.
- W570018495 cites W2129904159 @default.
- W570018495 cites W2130552816 @default.
- W570018495 cites W2134538788 @default.
- W570018495 cites W2137673278 @default.
- W570018495 cites W2141711186 @default.
- W570018495 cites W2145710490 @default.
- W570018495 cites W2151406283 @default.
- W570018495 cites W2151665739 @default.
- W570018495 cites W2152139261 @default.
- W570018495 cites W2152908685 @default.
- W570018495 cites W2153493310 @default.
- W570018495 cites W2155661248 @default.
- W570018495 cites W2157759988 @default.
- W570018495 cites W2162169970 @default.
- W570018495 cites W2163174294 @default.
- W570018495 cites W2164174130 @default.
- W570018495 cites W2168375114 @default.
- W570018495 cites W2395431563 @default.
- W570018495 doi "https://doi.org/10.1016/j.bbamcr.2015.05.005" @default.
- W570018495 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25962623" @default.
- W570018495 hasPublicationYear "2015" @default.
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