Matches in SemOpenAlex for { <https://semopenalex.org/work/W570850711> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W570850711 abstract "This thesis describes studies on the binding of peptides to the murine majorhistocompatibility complex (MHC) class I molecule H-2Db (Db). The expression of therecombinant soluble Db molecule in Chinese hamster ovary cells and its subsequentpurification by nickel affinity chromatography, gel filtration, and preparative nativeisoelectric focusing are reported. The product is the correct molecule, homogeneous, adimer of dimers, and free of endogenous peptide.A novel binding assay based on the enhancement of natural tryptophan fluorescence bythe binding of peptide is introduced. This assay is used to determine melting curves of theempty and peptide-loaded protein, and to measure association rate constants by stopped-flowfluorescence spectroscopy.Radioligand binding measurements of equilibrium as well as association and dissociationrate constants and their temperature dependence are reported. In agreement with earlierobservations, the ratio of association and dissociation rate constants is much larger thanthe equilibrium association constant.Fluorescence anisotropy decay spectroscopy gives evidence for conformationalalterations in the Db molecule upon peptide binding.The data, possible errors and ways to avoid them, and mathematical models of bindingare discussed to obtain an overall picture of the binding process." @default.
- W570850711 created "2016-06-24" @default.
- W570850711 creator A5058498996 @default.
- W570850711 date "1996-01-01" @default.
- W570850711 modified "2023-09-26" @default.
- W570850711 title "The biochemistry of antigen presentation" @default.
- W570850711 hasPublicationYear "1996" @default.
- W570850711 type Work @default.
- W570850711 sameAs 570850711 @default.
- W570850711 citedByCount "0" @default.
- W570850711 crossrefType "dissertation" @default.
- W570850711 hasAuthorship W570850711A5058498996 @default.
- W570850711 hasConcept C10710636 @default.
- W570850711 hasConcept C107824862 @default.
- W570850711 hasConcept C111357860 @default.
- W570850711 hasConcept C121332964 @default.
- W570850711 hasConcept C147789679 @default.
- W570850711 hasConcept C148898269 @default.
- W570850711 hasConcept C158711907 @default.
- W570850711 hasConcept C170493617 @default.
- W570850711 hasConcept C175656101 @default.
- W570850711 hasConcept C178790620 @default.
- W570850711 hasConcept C181199279 @default.
- W570850711 hasConcept C185592680 @default.
- W570850711 hasConcept C193363816 @default.
- W570850711 hasConcept C2779281246 @default.
- W570850711 hasConcept C2779546866 @default.
- W570850711 hasConcept C32786932 @default.
- W570850711 hasConcept C32909587 @default.
- W570850711 hasConcept C41625074 @default.
- W570850711 hasConcept C50476208 @default.
- W570850711 hasConcept C55493867 @default.
- W570850711 hasConcept C62520636 @default.
- W570850711 hasConcept C71240020 @default.
- W570850711 hasConcept C74998103 @default.
- W570850711 hasConcept C8010536 @default.
- W570850711 hasConcept C91881484 @default.
- W570850711 hasConcept C93391505 @default.
- W570850711 hasConceptScore W570850711C10710636 @default.
- W570850711 hasConceptScore W570850711C107824862 @default.
- W570850711 hasConceptScore W570850711C111357860 @default.
- W570850711 hasConceptScore W570850711C121332964 @default.
- W570850711 hasConceptScore W570850711C147789679 @default.
- W570850711 hasConceptScore W570850711C148898269 @default.
- W570850711 hasConceptScore W570850711C158711907 @default.
- W570850711 hasConceptScore W570850711C170493617 @default.
- W570850711 hasConceptScore W570850711C175656101 @default.
- W570850711 hasConceptScore W570850711C178790620 @default.
- W570850711 hasConceptScore W570850711C181199279 @default.
- W570850711 hasConceptScore W570850711C185592680 @default.
- W570850711 hasConceptScore W570850711C193363816 @default.
- W570850711 hasConceptScore W570850711C2779281246 @default.
- W570850711 hasConceptScore W570850711C2779546866 @default.
- W570850711 hasConceptScore W570850711C32786932 @default.
- W570850711 hasConceptScore W570850711C32909587 @default.
- W570850711 hasConceptScore W570850711C41625074 @default.
- W570850711 hasConceptScore W570850711C50476208 @default.
- W570850711 hasConceptScore W570850711C55493867 @default.
- W570850711 hasConceptScore W570850711C62520636 @default.
- W570850711 hasConceptScore W570850711C71240020 @default.
- W570850711 hasConceptScore W570850711C74998103 @default.
- W570850711 hasConceptScore W570850711C8010536 @default.
- W570850711 hasConceptScore W570850711C91881484 @default.
- W570850711 hasConceptScore W570850711C93391505 @default.
- W570850711 hasLocation W5708507111 @default.
- W570850711 hasOpenAccess W570850711 @default.
- W570850711 hasPrimaryLocation W5708507111 @default.
- W570850711 hasRelatedWork W1532231986 @default.
- W570850711 hasRelatedWork W1995325539 @default.
- W570850711 hasRelatedWork W1997729144 @default.
- W570850711 hasRelatedWork W2001839423 @default.
- W570850711 hasRelatedWork W2002013931 @default.
- W570850711 hasRelatedWork W2009629056 @default.
- W570850711 hasRelatedWork W2019926384 @default.
- W570850711 hasRelatedWork W2037500120 @default.
- W570850711 hasRelatedWork W2046293692 @default.
- W570850711 hasRelatedWork W2048118889 @default.
- W570850711 hasRelatedWork W2059407788 @default.
- W570850711 hasRelatedWork W2077415084 @default.
- W570850711 hasRelatedWork W2119623049 @default.
- W570850711 hasRelatedWork W2122528791 @default.
- W570850711 hasRelatedWork W2131439250 @default.
- W570850711 hasRelatedWork W2141053295 @default.
- W570850711 hasRelatedWork W2509115276 @default.
- W570850711 hasRelatedWork W2732840194 @default.
- W570850711 hasRelatedWork W2744673655 @default.
- W570850711 hasRelatedWork W3093192010 @default.
- W570850711 isParatext "false" @default.
- W570850711 isRetracted "false" @default.
- W570850711 magId "570850711" @default.
- W570850711 workType "dissertation" @default.