Matches in SemOpenAlex for { <https://semopenalex.org/work/W57128600> ?p ?o ?g. }
- W57128600 endingPage "2299" @default.
- W57128600 startingPage "2293" @default.
- W57128600 abstract "Objective: Acute lung injury in Pseudomonas aeruginosa pneumonia depends primarily on ExoU toxin being delivered directly into the eukaryotic cell cytosol through the type III secretion system. The amino-acid sequence of ExoU has a potato patatin-like phospholipase domain, similar to the sequence of mammalian Ca2+-independent phospholipase A2. We examined whether the acute lung injury caused by cytotoxic P. aeruginosa was dependent on the patatin-like phospholipase domain of ExoU. Design: Laboratory investigation using an established mouse model for P. aeruginosa pneumonia with quantitative measurements of acute lung injury and mortality. Setting: University experimental research laboratory. Subjects: Balb/c mice. Interventions: First, a site-directional mutation was introduced in the predicted catalytically active site of the patatin-like phospholipase domain of recombinant ExoU protein. The effect of the mutation on the catalytic activity of ExoU was tested by the in vitro lysophospholipase A assay. Second, the same site-directional mutation was introduced into the exoU gene of P. aeruginosa PA103. Mice were intratracheally infected with either a wild-type P. aeruginosa strain PA103 or an isogenic mutant containing the mutation in exoU. Acute epithelial lung injury, lung edema, bacteremia, and mortality were evaluated quantitatively. Measurements and Main Results: Recombinant ExoU had lysophospholipase A activity. Site-directional mutations in the predicted catalytic site of ExoU caused a loss of the lysophospholipase A activity. Whereas the airspace instillation of PA103 caused acute lung injury and death of the infected mice, the airspace instillation of isogenic mutants secreting catalytically inactive ExoU were noncytotoxic and did not cause acute lung injury or death of the infected mice. Conclusion: Virulent P. aeruginosa causes acute lung injury and death by the cytotoxic activity derived from the patatin-like phospholipase domain of ExoU." @default.
- W57128600 created "2016-06-24" @default.
- W57128600 creator A5013706986 @default.
- W57128600 creator A5019471778 @default.
- W57128600 creator A5031601538 @default.
- W57128600 creator A5033935101 @default.
- W57128600 creator A5038214218 @default.
- W57128600 creator A5040862910 @default.
- W57128600 creator A5087550018 @default.
- W57128600 date "2004-11-01" @default.
- W57128600 modified "2023-10-17" @default.
- W57128600 title "Pseudomonas aeruginosa causes acute lung injury via the catalytic activity of the patatin-like phospholipase domain of ExoU*" @default.
- W57128600 cites W1509650469 @default.
- W57128600 cites W1511786918 @default.
- W57128600 cites W1537917457 @default.
- W57128600 cites W1551381812 @default.
- W57128600 cites W1763142230 @default.
- W57128600 cites W1917030361 @default.
- W57128600 cites W1969290128 @default.
- W57128600 cites W1982732431 @default.
- W57128600 cites W1986311330 @default.
- W57128600 cites W1990299056 @default.
- W57128600 cites W1990691268 @default.
- W57128600 cites W2004668209 @default.
- W57128600 cites W2005248681 @default.
- W57128600 cites W2030601577 @default.
- W57128600 cites W2031703416 @default.
- W57128600 cites W2036221715 @default.
- W57128600 cites W2058246783 @default.
- W57128600 cites W2064341721 @default.
- W57128600 cites W2068773073 @default.
- W57128600 cites W2077049678 @default.
- W57128600 cites W2087967484 @default.
- W57128600 cites W2088927230 @default.
- W57128600 cites W2091955762 @default.
- W57128600 cites W2096985979 @default.
- W57128600 cites W2106006268 @default.
- W57128600 cites W2111724800 @default.
- W57128600 cites W2119420633 @default.
- W57128600 cites W2120339843 @default.
- W57128600 cites W2132305492 @default.
- W57128600 cites W2137749186 @default.
- W57128600 cites W2144878722 @default.
- W57128600 cites W2146388445 @default.
- W57128600 cites W2150637507 @default.
- W57128600 cites W2150704159 @default.
- W57128600 cites W2155511306 @default.
- W57128600 cites W2158102238 @default.
- W57128600 cites W2158714788 @default.
- W57128600 cites W2161014327 @default.
- W57128600 cites W2228337857 @default.
- W57128600 cites W4211023012 @default.
- W57128600 doi "https://doi.org/10.1097/01.ccm.0000145588.79063.07" @default.
- W57128600 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15640644" @default.
- W57128600 hasPublicationYear "2004" @default.
- W57128600 type Work @default.
- W57128600 sameAs 57128600 @default.
- W57128600 citedByCount "71" @default.
- W57128600 countsByYear W571286002012 @default.
- W57128600 countsByYear W571286002014 @default.
- W57128600 countsByYear W571286002015 @default.
- W57128600 countsByYear W571286002016 @default.
- W57128600 countsByYear W571286002017 @default.
- W57128600 countsByYear W571286002018 @default.
- W57128600 countsByYear W571286002019 @default.
- W57128600 countsByYear W571286002020 @default.
- W57128600 countsByYear W571286002021 @default.
- W57128600 countsByYear W571286002022 @default.
- W57128600 countsByYear W571286002023 @default.
- W57128600 crossrefType "journal-article" @default.
- W57128600 hasAuthorship W57128600A5013706986 @default.
- W57128600 hasAuthorship W57128600A5019471778 @default.
- W57128600 hasAuthorship W57128600A5031601538 @default.
- W57128600 hasAuthorship W57128600A5033935101 @default.
- W57128600 hasAuthorship W57128600A5038214218 @default.
- W57128600 hasAuthorship W57128600A5040862910 @default.
- W57128600 hasAuthorship W57128600A5087550018 @default.
- W57128600 hasConcept C104317684 @default.
- W57128600 hasConcept C12927208 @default.
- W57128600 hasConcept C143065580 @default.
- W57128600 hasConcept C181199279 @default.
- W57128600 hasConcept C2776551241 @default.
- W57128600 hasConcept C2776888444 @default.
- W57128600 hasConcept C2777637488 @default.
- W57128600 hasConcept C2778530336 @default.
- W57128600 hasConcept C523546767 @default.
- W57128600 hasConcept C54355233 @default.
- W57128600 hasConcept C55493867 @default.
- W57128600 hasConcept C71924100 @default.
- W57128600 hasConcept C86803240 @default.
- W57128600 hasConcept C89423630 @default.
- W57128600 hasConceptScore W57128600C104317684 @default.
- W57128600 hasConceptScore W57128600C12927208 @default.
- W57128600 hasConceptScore W57128600C143065580 @default.
- W57128600 hasConceptScore W57128600C181199279 @default.
- W57128600 hasConceptScore W57128600C2776551241 @default.
- W57128600 hasConceptScore W57128600C2776888444 @default.
- W57128600 hasConceptScore W57128600C2777637488 @default.