Matches in SemOpenAlex for { <https://semopenalex.org/work/W571786864> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W571786864 endingPage "1038" @default.
- W571786864 startingPage "1027" @default.
- W571786864 abstract "Abstract Bacillus methanolicus MGA3 is a Gram-positive aerobic methylotroph growing optimally at 50–53 °C. Methylotrophy in B. methanolicus is encoded on pBM19 and by two chromosomal copies of the methanol dehydrogenase (mdh), hexulose phosphate synthase (hps) and phosphohexuloisomerase (phi) genes. However, there are no published studies on the regulation of methylotrophy or the dominant mechanism of detoxification of intracellular formaldehyde in response to high methanol concentration. The µ max of B. methanolicus MGA3 was assessed on methanol, mannitol and glucose. B. methanolicus achieved a µ max at 25 mM initial methanol of 0.65 ± 0.007 h−1, which decreased to 0.231 ± 0.004 h−1 at 2 M initial methanol. Slow growth was also observed with initial methanol concentrations of >2 M. The µ max on mannitol and glucose are 0.532 ± 0.002 and 0.336 ± 0.003 h−1, respectively. Spiking cultures with additional methanol (100 mM) did not disturb the growth rate of methanol-grown cells, whereas, a 50 mM methanol spike halted the growth in mannitol. Surprisingly, growth in methanol was inhibited by 1 mM formaldehyde, while mannitol-grown cells tolerated 2 mM. Moreover, mannitol-grown cells removed formaldehyde faster than methanol-grown cells. Further, we show that methanol oxidation in B. methanolicus MGA3 is mainly carried out by the pBM19-encoded mdh. Formaldehyde and formate addition down-regulate the mdh and hps genes in methanol-grown cells. Similarly, they down-regulate mdh genes in mannitol-grown cells, but up-regulate hps. Phosphofructokinase (pfk) is up-regulated in both methanol and mannitol-grown cells, which suggests that pfk may be a possible synthetic methylotrophy target to reduce formaldehyde growth toxicity at high methanol concentrations." @default.
- W571786864 created "2016-06-24" @default.
- W571786864 creator A5014409005 @default.
- W571786864 creator A5065386169 @default.
- W571786864 creator A5070300220 @default.
- W571786864 date "2015-07-01" @default.
- W571786864 modified "2023-10-03" @default.
- W571786864 title "Growth of <i>Bacillus methanolicus</i> in 2 M methanol at 50 °C: the effect of high methanol concentration on gene regulation of enzymes involved in formaldehyde detoxification by the ribulose monophosphate pathway" @default.
- W571786864 cites W1607928695 @default.
- W571786864 cites W1983086586 @default.
- W571786864 cites W1987959868 @default.
- W571786864 cites W1991242334 @default.
- W571786864 cites W1991479295 @default.
- W571786864 cites W2025545461 @default.
- W571786864 cites W2027496130 @default.
- W571786864 cites W2049048597 @default.
- W571786864 cites W2051815879 @default.
- W571786864 cites W2075159857 @default.
- W571786864 cites W2076028576 @default.
- W571786864 cites W2076336907 @default.
- W571786864 cites W2084333783 @default.
- W571786864 cites W2087828239 @default.
- W571786864 cites W2108268803 @default.
- W571786864 cites W2112949067 @default.
- W571786864 cites W2136911633 @default.
- W571786864 cites W2145174745 @default.
- W571786864 cites W2149114272 @default.
- W571786864 cites W2157312398 @default.
- W571786864 cites W4211152560 @default.
- W571786864 cites W4319291076 @default.
- W571786864 cites W44183036 @default.
- W571786864 doi "https://doi.org/10.1007/s10295-015-1623-8" @default.
- W571786864 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25952117" @default.
- W571786864 hasPublicationYear "2015" @default.
- W571786864 type Work @default.
- W571786864 sameAs 571786864 @default.
- W571786864 citedByCount "10" @default.
- W571786864 countsByYear W5717868642015 @default.
- W571786864 countsByYear W5717868642016 @default.
- W571786864 countsByYear W5717868642018 @default.
- W571786864 countsByYear W5717868642019 @default.
- W571786864 countsByYear W5717868642020 @default.
- W571786864 countsByYear W5717868642021 @default.
- W571786864 countsByYear W5717868642022 @default.
- W571786864 crossrefType "journal-article" @default.
- W571786864 hasAuthorship W571786864A5014409005 @default.
- W571786864 hasAuthorship W571786864A5065386169 @default.
- W571786864 hasAuthorship W571786864A5070300220 @default.
- W571786864 hasConcept C153911025 @default.
- W571786864 hasConcept C178790620 @default.
- W571786864 hasConcept C181199279 @default.
- W571786864 hasConcept C185592680 @default.
- W571786864 hasConcept C2775930285 @default.
- W571786864 hasConcept C2777281465 @default.
- W571786864 hasConcept C2778445172 @default.
- W571786864 hasConcept C2778973328 @default.
- W571786864 hasConcept C2779607525 @default.
- W571786864 hasConcept C2779617599 @default.
- W571786864 hasConcept C538909803 @default.
- W571786864 hasConcept C55493867 @default.
- W571786864 hasConcept C86803240 @default.
- W571786864 hasConceptScore W571786864C153911025 @default.
- W571786864 hasConceptScore W571786864C178790620 @default.
- W571786864 hasConceptScore W571786864C181199279 @default.
- W571786864 hasConceptScore W571786864C185592680 @default.
- W571786864 hasConceptScore W571786864C2775930285 @default.
- W571786864 hasConceptScore W571786864C2777281465 @default.
- W571786864 hasConceptScore W571786864C2778445172 @default.
- W571786864 hasConceptScore W571786864C2778973328 @default.
- W571786864 hasConceptScore W571786864C2779607525 @default.
- W571786864 hasConceptScore W571786864C2779617599 @default.
- W571786864 hasConceptScore W571786864C538909803 @default.
- W571786864 hasConceptScore W571786864C55493867 @default.
- W571786864 hasConceptScore W571786864C86803240 @default.
- W571786864 hasIssue "7" @default.
- W571786864 hasLocation W5717868641 @default.
- W571786864 hasLocation W5717868642 @default.
- W571786864 hasOpenAccess W571786864 @default.
- W571786864 hasPrimaryLocation W5717868641 @default.
- W571786864 hasRelatedWork W1507344589 @default.
- W571786864 hasRelatedWork W1992525248 @default.
- W571786864 hasRelatedWork W1994658822 @default.
- W571786864 hasRelatedWork W1994682802 @default.
- W571786864 hasRelatedWork W2007920582 @default.
- W571786864 hasRelatedWork W2029787939 @default.
- W571786864 hasRelatedWork W2073246986 @default.
- W571786864 hasRelatedWork W2395046770 @default.
- W571786864 hasRelatedWork W2615584870 @default.
- W571786864 hasRelatedWork W2885245566 @default.
- W571786864 hasVolume "42" @default.
- W571786864 isParatext "false" @default.
- W571786864 isRetracted "false" @default.
- W571786864 magId "571786864" @default.
- W571786864 workType "article" @default.