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- W574227777 abstract "A culture system for the maintenance of embryonic mouse brain cells, in vitro, was established. The cells in these cultures were characterized by histological, biochemical and electrophysiological techniques. It was shown that although some neurons were capable of some proliferation in vitro, the non-neuronal cells actively proliferated and eventually dominated the cultures. Cytotoxic nucleoside analogues were used to prevent overgrowth and select for differentiated neurons, and it was shown that arabinofuranosylcytosine was particularly effective in this respect. The culture system was used to examine the interrelationships of radiation survival, DNA replication, DNA repair and viral expression with respect to differentiation.Radiosensitivity of neuronal and non-neuronal cells was compared. Non-neuronal cells were more sensitive and this sensitivity was related to their proliferative capacity.Unscheduled DNA synthesis in response to ultraviolet light was examined. It was shown that a large proportion of neurons did not exhibit repair labelling and apparent levels of incorporation in labelled neurons was lower than in non-neuronal cells. These experiments were performed with autoradiography of tritiated-thymidine labelled cells : when corrections were made for autoradiographic efficiency, apparent levels of repair incorporation were equal in differentiated neurons and non-neuronal cells. The penetration of ultraviolet light in cells was examined and it was shown that although cells showed a limited transmission of ultraviolet light, the effects of this on assays were outweighed by the poor penetration of tritium beta-particles. The significance of these results in terms of previous studies reported in the literature was discussed.The expression of the papovaviruses, SV40 and polyoma, was examined in brain cultures. A comparison of normal and arabinofuranosylcytosine treated cultures indicated a preference for proliferating cells, with differentiated neurons being the most resistant. This was confirmed with SV40, by simultaneous cytological detection of viral antigen and cellular DNA replication in individual cells. DNA replication status was shown to be an important factor in controlling virus expression. The levels at which this control may be exerted were discussed." @default.
- W574227777 created "2016-06-24" @default.
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- W574227777 date "2014-12-08" @default.
- W574227777 modified "2023-09-24" @default.
- W574227777 title "DNA repair: replication and viral expression in mouse brain cell cultures" @default.
- W574227777 doi "https://doi.org/10.14264/uql.2014.539" @default.
- W574227777 hasPublicationYear "2014" @default.
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