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- W57429085 abstract "Publisher Summary This chapter describes the digitonin method of permeabilizing gastric parietal cells using isolated rabbit gastric glands and isolated unpurified rabbit gastric cells (which are both heterogeneous cell populations, containing mainly parietal and peptic cells) as well as purified parietal cells. Digitonin-permeabilized purified parietal cells, which retain their functional state, will prove to be a useful model to study cellular mechanisms regulating acid secretion as well as properties of the H + ,K + -ATPase in situ , without interference from other cell types. Two methods of assessing the degree of permeabilization and a method to assess parietal cell functional state will also be described in this chapter. Recently, digitonin was successfully used to permeabilize the apical or mucosal membrane of colonic epithelial sheets, which were mounted in Ussing chambers, allowing the study of the properties of the K + and CI - conductances of the basal lateral membrane. Thus, digitonin permeabilization is becoming a widely used and important technique in studies of intracellular mechanisms and membrane transport." @default.
- W57429085 created "2016-06-24" @default.
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- W57429085 date "1990-01-01" @default.
- W57429085 modified "2023-09-25" @default.
- W57429085 title "[7] Permeabilizing parietal cells" @default.
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- W57429085 doi "https://doi.org/10.1016/0076-6879(90)92065-l" @default.
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