Matches in SemOpenAlex for { <https://semopenalex.org/work/W57432843> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W57432843 endingPage "71" @default.
- W57432843 startingPage "63" @default.
- W57432843 abstract "Site-directed mutagenesis or directed evolution of proteins often leads to the production of inactive mutants. For streptavidin and related proteins, mutations may lead to the loss of their biotin-binding properties. With high-throughput screening methodologies in mind, it is imperative to detect, prior to the high-density protein production, the bacteria that produce non-functional streptavidin isoforms. Based on the incorporation of biotin-4-fluorescein in streptavidin mutants present in Escherichia coli bacterial extracts, we detail a functional screen that allows the identification of biotin-binding streptavidin variants. Bacteria are cultivated in a small volume, followed by a rapid treatment of the cells; biotin-4-fluorescein is added to the bacterial extract and loaded on an Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis (SDS–PAGE) under non-denaturing conditions. Revealing is performed using a UV transilluminator. This screen is thus easy to implement, cheap and requires only readily available equipment." @default.
- W57432843 created "2016-06-24" @default.
- W57432843 creator A5057806953 @default.
- W57432843 creator A5084113419 @default.
- W57432843 date "2008-01-01" @default.
- W57432843 modified "2023-09-23" @default.
- W57432843 title "Functionality Screen of Streptavidin Mutants by Non-Denaturing SDS–PAGE Using Biotin-4-Fluorescein" @default.
- W57432843 cites W1898560826 @default.
- W57432843 cites W1980693947 @default.
- W57432843 cites W1986899975 @default.
- W57432843 cites W2010264074 @default.
- W57432843 cites W2021762142 @default.
- W57432843 cites W2037576778 @default.
- W57432843 cites W2043936750 @default.
- W57432843 cites W2044195182 @default.
- W57432843 cites W2056159899 @default.
- W57432843 cites W2079651052 @default.
- W57432843 cites W2082948336 @default.
- W57432843 cites W2098912166 @default.
- W57432843 cites W2100837269 @default.
- W57432843 cites W2111410489 @default.
- W57432843 cites W2121351614 @default.
- W57432843 cites W2127664963 @default.
- W57432843 cites W2397979107 @default.
- W57432843 cites W4211027922 @default.
- W57432843 doi "https://doi.org/10.1007/978-1-59745-579-4_6" @default.
- W57432843 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18287650" @default.
- W57432843 hasPublicationYear "2008" @default.
- W57432843 type Work @default.
- W57432843 sameAs 57432843 @default.
- W57432843 citedByCount "0" @default.
- W57432843 crossrefType "book-chapter" @default.
- W57432843 hasAuthorship W57432843A5057806953 @default.
- W57432843 hasAuthorship W57432843A5084113419 @default.
- W57432843 hasConcept C104317684 @default.
- W57432843 hasConcept C116084860 @default.
- W57432843 hasConcept C121332964 @default.
- W57432843 hasConcept C143065580 @default.
- W57432843 hasConcept C153911025 @default.
- W57432843 hasConcept C16318435 @default.
- W57432843 hasConcept C185592680 @default.
- W57432843 hasConcept C204628709 @default.
- W57432843 hasConcept C2776190903 @default.
- W57432843 hasConcept C2778015030 @default.
- W57432843 hasConcept C2778204606 @default.
- W57432843 hasConcept C2778913249 @default.
- W57432843 hasConcept C55493867 @default.
- W57432843 hasConcept C62520636 @default.
- W57432843 hasConcept C86803240 @default.
- W57432843 hasConcept C91881484 @default.
- W57432843 hasConceptScore W57432843C104317684 @default.
- W57432843 hasConceptScore W57432843C116084860 @default.
- W57432843 hasConceptScore W57432843C121332964 @default.
- W57432843 hasConceptScore W57432843C143065580 @default.
- W57432843 hasConceptScore W57432843C153911025 @default.
- W57432843 hasConceptScore W57432843C16318435 @default.
- W57432843 hasConceptScore W57432843C185592680 @default.
- W57432843 hasConceptScore W57432843C204628709 @default.
- W57432843 hasConceptScore W57432843C2776190903 @default.
- W57432843 hasConceptScore W57432843C2778015030 @default.
- W57432843 hasConceptScore W57432843C2778204606 @default.
- W57432843 hasConceptScore W57432843C2778913249 @default.
- W57432843 hasConceptScore W57432843C55493867 @default.
- W57432843 hasConceptScore W57432843C62520636 @default.
- W57432843 hasConceptScore W57432843C86803240 @default.
- W57432843 hasConceptScore W57432843C91881484 @default.
- W57432843 hasLocation W574328431 @default.
- W57432843 hasLocation W574328432 @default.
- W57432843 hasOpenAccess W57432843 @default.
- W57432843 hasPrimaryLocation W574328431 @default.
- W57432843 hasRelatedWork W1511280725 @default.
- W57432843 hasRelatedWork W1876747049 @default.
- W57432843 hasRelatedWork W1984229904 @default.
- W57432843 hasRelatedWork W1989126998 @default.
- W57432843 hasRelatedWork W2010264074 @default.
- W57432843 hasRelatedWork W2070390193 @default.
- W57432843 hasRelatedWork W2070869769 @default.
- W57432843 hasRelatedWork W2396522900 @default.
- W57432843 hasRelatedWork W57432843 @default.
- W57432843 hasRelatedWork W945742090 @default.
- W57432843 isParatext "false" @default.
- W57432843 isRetracted "false" @default.
- W57432843 magId "57432843" @default.
- W57432843 workType "book-chapter" @default.