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- W57482685 abstract "The present study was to isolate VRE from imported beef Tn Malaysia, beef is themajor consuming animal originated food and most of the beef is imported from thosecountries where the use of antibiotics in the feed of animals as a growth promoter was acommon practice and was licensed. Out of 150 samples, 17 (11.3%) were positive forVRE. Sixty-seven (67) VRE were isolated from frozen imported beef (48) and burgers(19). The species identified were E. faeclum (35), E. faecalls (22), E. faecallsasaccharolytic variant (3), E. pseudoavlum (3), E. gal/marum (2), E. maldoratus (1) andE. avlum (l). Various plating media and broths were evaluated for the isolation of VRE.Azide Dextrose broth (ADB) with vancomycin concentration of 50 µg/ml for 48 henrichment and plating on Slanetz and Bartley agar (SBA) with vancomycinconcentration of 50 µg/ml was concluded best for isolation of VRE. In the present studyantibiotic resistance patterns and the rates of resistance of 67 isolates were evaluated. Itwas observed that all the isolates were multiple resistant and resistant to ten of the sixteenantibiotics tested. All isolates were 100% resistant to streptomycin, vancomycin andteicoplanin. Other isolates were resistant between 94% to 97% to other eight antibiotics.Penicillin, ampicil1in and chloremphenicol showed the least resistance namely, 26.8, 38.8and 58.2%, respectively. Hemolytic activity on horse blood agar showed that 29 out of 67isolates (43.3%) were j3-hemolytic indicating to have potency to be pathogenic. Theplasmid profiling revealed that 39 (58.2%) out of 67 bear plasmids of the range 1.0 to35.8 MDa. Using specific PCR, vanA gene was detected among 65 of 67 isolates (97%)which is considered to make these isolates resistant to vancomycin. The molecularepidemiology of E. faecium and E. faecalis using RAPD-PCR technique showed thedifference in the genetic relatedness of the strains isolated from frozen imported beef andbeef burgers. It showed the genetic relatedness in terms of % similarity from thedendrogram prepared between all the strains taken into study_ RAPD-PCR gave highdiscriminating results between all the strains. The work clearly reveals that beef can be avehicle for VRE in Malaysia. The need for intervention to control or eliminate antibioticresistant Enlerococcu') from foods of animal origin has been made clearer by the resultspresented in this study." @default.
- W57482685 created "2016-06-24" @default.
- W57482685 creator A5072091883 @default.
- W57482685 date "2001-01-01" @default.
- W57482685 modified "2023-09-23" @default.
- W57482685 title "Prevalence and Molecular Characterisation of Vancomycin Resistant Enterococci (Vre) Isolated from Beef" @default.
- W57482685 hasPublicationYear "2001" @default.
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