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- W57492812 abstract "Resistance to chemical insecticides has been described to a vast order of insectsand represents one of the greatest challenges for obtaining success in controlprograms of disease vectors. Resistance mechanisms to chemical insecticides canbe divided in categories, including target-site insensitivity and metabolicmechanisms, through the expression of the organism detoxifying enzymes, suchas esterases. At the moment, esterases have been identified as the mainmechanism conferring resistance to organophosphates in Aedes aegypti. The mainobjective of this study was the analysis of the esterase genetic polymorphism and,to evaluate its role in possible mechanisms of resistance to temephos in naturalpopulations of this mosquito. To answer these questions, we used two laboratorystrains: Rockefeller (susceptibility model for all the insecticides groups) and theRecife-Resistente (kept under strong selective pressure by temephos for fivegenerations), and nine natural populations: eight from the metropolitan area ofRecife and one from Araripe (CE). These populations were collected as eggs,during the years 2004 and 2005. It was performed techniques such as biochemicalassays, isozymes electrophoresis, PCR and sequencing of part of the gene, andReal-Time PCR to compare the amount of gene copies of the gene in a resistantand a susceptible strain, and in natural populations. Biochemical assays were runonly in Recife-Resistente strain and, the results showed the presence of themetabolic mechanism of resistance, through high esterase activity. The esterasespattern was observed in nine populations, in 6% polyacrylamide gel stained withspecific substrates to α and β-esterase enzymes. The observed heterozygosityvalues (Ho) varied from 0,278 to 0,533 in 2004, and in 2005, from 0,388 to 0,608.The gels also showed a region of high esterase activity, denominated locus 1. Inthis locus, the allele responsible for the higher esterase activity was called 3. Itsfrequency varied in 2004 from 0,100 in Dois Irmaos to 0,191 in Alto Jose do Pinho,and in 2005, dropped to 0,071 and 0,107 respectively. In the Recife-Resistentestrain, the frequency of this allele was 0,214. The sequencing of part of exon 4from the α-esterase gene showed that the analyzed fragment, approximately 180pb long, is quite conserved, presenting only four polymorphic sites. The resultsobtained by Real-Time PCR indicated that the populations submitted to the studydid not present gene amplification to the α-esterase gene, when compared toRockefeller strain, except the populations from Engenho do Meio e Araripe. Inspiteof the higher number of molecules presented by these two populations, the resultsstill need more proofs to guarantee that the superexpression of the gene is thepossible cause of the resistance. The frequency of the superexpressed allele canbe monitored through time and help the management of resistance to chemicalinsecticides in field projects" @default.
- W57492812 created "2016-06-24" @default.
- W57492812 creator A5003178305 @default.
- W57492812 date "2006-01-01" @default.
- W57492812 modified "2023-09-23" @default.
- W57492812 title "Monitoramento do gene, que codifica a esterase, envolvido na resistência a inseticidas organofosforados em populações naturais de Aedes aegypti do Brasil" @default.
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