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- W576156707 abstract "In order to prepare large-scale of the natural active soluble canine transferrin receptor(sTfR),the sTfR gene codons were optimized to increase sTfR expression level in eukaryotic cells.The gene encoding sTfR was amplified from canine liver tissues by RT-PCR.According to amino acid sequence,sTfR codons were optimized based on human main codons and synthesized.The eukaryotic expression vectors of wild-type and codon-optimized sTfR genes were construced by using pcDNA3.1-CD5 vector.The vectors were transfected into human embryonic kidney cells HEK293T cells mediated by calcium phosphate.The expressed sTfR proteins were identified by Western-blot.Binding activity of sTfR to canine parvovirus VP2 protein was analyzed by ELISA.The results showed that the amplified sTfR gene sequence was 100% similarity to the published in GenBank;The expression level of codon-optimized sTfR gene showed an increase of 75% compared with wild-type in 293T cells;sTfR protein was proved to have the specific binding activity for canine parvovirus VP2 protein." @default.
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- W576156707 date "2009-01-01" @default.
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- W576156707 title "Study on codon optimization and expression of canine sTfR gene in eukaryotic cells and interaction with canine parvovirus VP2 protein" @default.
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