FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W578594930> ?p ?o ?g. }

• W578594930 abstract "Tendon injuries of the hand are common with nearly one-third of a million digital flexor tendon injuries per year in the United States. Injuries in zone II of the flexor tendon are notoriously difficult to repair and the main complications are either tendon rupture or adhesion formation. Adhesions remain a problem despite many attempts at prevention using various chemicals and physical barrier techniques. The overall aim of this thesis was to further understand the biology of tendon adhesion formation and to develop novel treatments targeting this process.Uninjured flexor tendons were obtained from New Zealand White rabbits. Tenocytes derived from different parts of the flexor tendon-sheath complex were grown using standard tissue culture techniques. Each of the three different cell types (endotenon, epitenon and tendon sheath) was subjected to various assays (proliferation/toxicology, cell adhesion, and mRNA expression,) using TGF-β1 and our proposed treatments; epigallocatechin-3-gallate (EGCG), Resveratrol and Pumactant.A further study then compared the three treatments in vivo. New Zealand White rabbits (n=8 per group; 32 in total) were anaesthetised and the flexor digitorum profundus (FDP) of digits 2 and 4 of the forepaw was subjected to a partial tenotomy. The three treatments (compared with control groups) were infiltrated into the flexor sheath of immobilised tendons and the wound was then sutured closed. After two weeks the tendons were harvested and randomised to either mechanical or histological assessment of adhesion formation.The major findings from the in vitro study were as follows: TGF-β1 showed a statistically significant increase in collagen type I gene expression in epitenon cells at 24 and 48 hours and an increase in collagen type III in sheath cells between 6 and 24 hours. There was a statistically significant down-regulation of collagen type III in endotenon and epitenon cells at various time points. Resveratrol showed a statistically significant increase in collagen type I gene expression in epitenon cells with a corresponding down-regulation of fibronectin and PAI-1 in both epitenon and sheath cells. Resveratrol also up-regulated collagen type III at late time points in tendon sheath cells. Pumactant also showed some therapeutic advantages at the gene expression level with a statistically significant increase in collagen type III in endotenon cells at late time points, corresponding with an overall down-regulation of PAI expression in the same cell type and sheath cells.The results from the in vivo study were that all three treatments showed a statistically significant reduction of tendon adhesion formation when compared to operated controls in both mechanical and histological assessments (p<0.05). However, Pumactant was the only treatment to demonstrate a statistically significant reduction in adhesion formation when compared to the H20-group using both methods (p<0.05).All three treatments displayed potential therapeutic advantages. However, Pumactant showed the most promising in vivo results and it would be worthwhile investigating this further with an in vivo model of tendon healing and if successful a pilot clinical trial. Hopefully this could act as a suitable adjunct to tendon repair in the future and improve the lives of patients with disabling tendon injuries." @default.
• W578594930 created "2016-06-24" @default.
• W578594930 creator A5025205550 @default.
• W578594930 date "2011-11-28" @default.
• W578594930 modified "2023-09-27" @default.
• W578594930 title "Novel therapeutics in the prevention of flexor tendon adhesion formation" @default.
• W578594930 cites W105558294 @default.
• W578594930 cites W109228634 @default.
• W578594930 cites W115255336 @default.
• W578594930 cites W1504191561 @default.
• W578594930 cites W1508850474 @default.
• W578594930 cites W1509166805 @default.
• W578594930 cites W1543068054 @default.
• W578594930 cites W1554566492 @default.
• W578594930 cites W1557506348 @default.
• W578594930 cites W1584682911 @default.
• W578594930 cites W1592102706 @default.
• W578594930 cites W1605158933 @default.
• W578594930 cites W1620029647 @default.
• W578594930 cites W16309918 @default.
• W578594930 cites W168593491 @default.
• W578594930 cites W1908415347 @default.
• W578594930 cites W1941535563 @default.
• W578594930 cites W1942307435 @default.
• W578594930 cites W1952271883 @default.
• W578594930 cites W1963554792 @default.
• W578594930 cites W1964108622 @default.
• W578594930 cites W1965660497 @default.
• W578594930 cites W1966482673 @default.
• W578594930 cites W1966975625 @default.
• W578594930 cites W1967419787 @default.
• W578594930 cites W1968375450 @default.
• W578594930 cites W1970596483 @default.
• W578594930 cites W1971039745 @default.
• W578594930 cites W1971959577 @default.
• W578594930 cites W1972880992 @default.
• W578594930 cites W1973461209 @default.
• W578594930 cites W1974935282 @default.
• W578594930 cites W1976535848 @default.
• W578594930 cites W1977013090 @default.
• W578594930 cites W1978011847 @default.
• W578594930 cites W1978759685 @default.
• W578594930 cites W1979064646 @default.
• W578594930 cites W1981992742 @default.
• W578594930 cites W1982018556 @default.
• W578594930 cites W1983173692 @default.
• W578594930 cites W1986002557 @default.
• W578594930 cites W1987314451 @default.
• W578594930 cites W1987372502 @default.
• W578594930 cites W1987477844 @default.
• W578594930 cites W1988245710 @default.
• W578594930 cites W1988969505 @default.
• W578594930 cites W1989079858 @default.
• W578594930 cites W1991210046 @default.
• W578594930 cites W1993821272 @default.
• W578594930 cites W1994946116 @default.
• W578594930 cites W1998799055 @default.
• W578594930 cites W1999098362 @default.
• W578594930 cites W1999209301 @default.
• W578594930 cites W1999359439 @default.
• W578594930 cites W1999375857 @default.
• W578594930 cites W1999735810 @default.
• W578594930 cites W2000224400 @default.
• W578594930 cites W2000272514 @default.
• W578594930 cites W2000400813 @default.
• W578594930 cites W2003651854 @default.
• W578594930 cites W2004277002 @default.
• W578594930 cites W2005930105 @default.
• W578594930 cites W2006124822 @default.
• W578594930 cites W2006946354 @default.
• W578594930 cites W2008146465 @default.
• W578594930 cites W2011015183 @default.
• W578594930 cites W2012991559 @default.
• W578594930 cites W2013610205 @default.
• W578594930 cites W2014379951 @default.
• W578594930 cites W2016583814 @default.
• W578594930 cites W2016750430 @default.
• W578594930 cites W2017686370 @default.
• W578594930 cites W2017781910 @default.
• W578594930 cites W2020225116 @default.
• W578594930 cites W2020305530 @default.
• W578594930 cites W2020506439 @default.
• W578594930 cites W2020556646 @default.
• W578594930 cites W2022347301 @default.
• W578594930 cites W2025642009 @default.
• W578594930 cites W2027352008 @default.
• W578594930 cites W2027558291 @default.
• W578594930 cites W2028453113 @default.
• W578594930 cites W202923795 @default.
• W578594930 cites W2031068056 @default.
• W578594930 cites W2034733039 @default.
• W578594930 cites W2035479325 @default.
• W578594930 cites W2036017101 @default.
• W578594930 cites W2036647753 @default.
• W578594930 cites W2036919864 @default.
• W578594930 cites W2038585308 @default.
• W578594930 cites W2038820009 @default.
• W578594930 cites W2040445124 @default.
• W578594930 cites W2040735530 @default.
• W578594930 cites W2040818311 @default.

• next