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- W579283729 abstract "The glutathione-S transferase(GST)gene of Fasiciola hepatica was cloned and expressed according to the sequences in GenBank and a pair of primers was prepared.The gene was amplified by RT-PCR and the DNA fragment was then cloned into vector pET-30a(+) and was induced in E.coli BL21(DE3) through IPTG induction.The expressed product was analyzed with SDS-PAGE and Western blotting.It was showed that the full-length of GST gene was 657 bp and its encoding product was 31.3 kDa in size,existing in the form of inclusion bodies.The recombinant protein expressed could induce efficiently the specific antibodies in SD rats,indicating its immunogenicity.In addition,the purified recombinant protein could be recognized specifically by serum of goats infected with Fasciola hepatica,demonstrating its immunogenicity." @default.
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- W579283729 date "2009-01-01" @default.
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- W579283729 title "Cloning and prokaryotic expression of glutathione-S transferase gene of Fasciola hepatica and the activity analysis of the recombinant protein." @default.
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