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• W57978968 abstract "The relationship between the rate of synthesis of nitric oxide (NO) and guanylate cyclase stimulation was used to characterize the kinetics of the NO synthase from rat forebrain and of some inhibitors of this enzyme. The NO synthase had an absolute requirement for L-arginine and NADPH and did not require any other cofactors. The enzyme had a Vmax. of 42 pmol of NO formed.min-1.mg of protein-1 and a Km for L-arginine of 8.4 microM. Three analogues of L-arginine, namely NG-monomethyl-L-arginine, NG-nitro-L-arginine and NG-iminoethyl-L-ornithine inhibited the brain NO synthase. All three compounds were competitive inhibitors of the enzyme with Ki values of 0.7, 0.4 and 1.2 microM respectively." @default.
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• W57978968 date "1990-07-01" @default.
• W57978968 modified "2023-10-16" @default.
• W57978968 title "Kinetic characteristics of nitric oxide synthase from rat brain" @default.
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• W57978968 doi "https://doi.org/10.1042/bj2690207" @default.
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