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- W580686282 abstract "The function of membrane receptors in the nervous system depends on physicochemical characteristics of neuronal membranes such as membrane order and phase. In this work, we have monitored the changes in hippocampal membrane order and related parameters by cholesterol and protein content utilizing a Nile Red-based phase-sensitive fluorescent membrane probe NR12S. Since alteration of membrane cholesterol is often associated with membrane phase change, the phase-sensitive nature of NR12S fluorescence becomes useful in these experiments. Our results show that fluorescence spectroscopic parameters such as emission maximum, anisotropy, and lifetime of NR12S display characteristic dependence on membrane cholesterol content. Interestingly, cholesterol-dependent red edge excitation shift is displayed by NR12S under these conditions. Hippocampal membranes exhibited reduction in liquid-ordered phase upon cholesterol depletion. These results provide insight into changes in hippocampal membrane order in the overall context of cholesterol and protein modulation." @default.
- W580686282 created "2016-06-24" @default.
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- W580686282 date "2015-08-01" @default.
- W580686282 modified "2023-10-16" @default.
- W580686282 title "Cholesterol-induced changes in hippocampal membranes utilizing a phase-sensitive fluorescence probe" @default.
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- W580686282 doi "https://doi.org/10.1016/j.bbamem.2015.05.001" @default.
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