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- W5808766 abstract "Third round fractions produced via bioactivity guided semi-preparative reversed phase HPLC fractionation of Echinacea angustifolia were analyzed by GC-MS to identify constituents important for the anti-inflammatory properties of the botanical. Two fractions were of interest due to their ability to inhibit lipopolysaccharide induced PGE2 production in RAW264.7 mouse macrophage cells and their GC-MS chromatograms having peaks for known and synthetically available compounds. When identification and quantification from GC-MS revealed that major constituents present in one fraction (1 µg/ml) were alkylamide 10 (0.15 µM) and ketone 23 (0.24 µM), and that the other fraction (1 µg/ml) consisted of alkylamide 11 (3.6 µM), these constituents were screened at the concentrations present in the fraction using the PGE2 assay. The combination of alkylamide 10 and ketone 23 did not explain the bioactivity of its respective fraction, but alkylamide 11 was partially explanatory of the bioactivity, accounting for 30% reduction of PGE2 production compared to 81% reduction of PGE2 identified with the fraction. Chemically synthesized ketones 21 and 23 were also shown to individually inhibit PGE2 and nitric oxide production at a concentration as low as 1µM, providing a basis to study these constituents further for anti-inflammatory potential. Funding: 9 P50 AT004155-06 from ODS/NCCAM, NIH. Grant Funding Source 9 P50 AT004155-06 from ODS/NCCAM, NIH" @default.
- W5808766 created "2016-06-24" @default.
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- W5808766 date "2009-04-01" @default.
- W5808766 modified "2023-09-24" @default.
- W5808766 title "Unraveling the complexity of Echinacea fractions to identify alkylamides and ketones important for anti‐inflammatory bioactivity" @default.
- W5808766 doi "https://doi.org/10.1096/fasebj.23.1_supplement.104.5" @default.
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