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- W58161231 abstract "A new gas chromatography (GC) method is presented for analysing both the conversion and the enantiomeric excess (e.e.) of samples from alcohol dehydrogenase reactions. The chiral compounds studied were a series of saturated, straight chain alcohols, ranging from 2-butanol to 2-heptanol. The alcohols were converted to the corresponding trifluoroacetylated derivatives by injecting trifluoroacetic anhydride onto the column shortly after injection of the aqueous samples in split-injection mode (1:100) onto a Chiraldex G-TA capillary GC column. Injecting seven hundred aqueous enzymatic reaction mixtures according to the above-mentioned procedure revealed no noticeable loss of column performance. Using the new GC method, conventional sample work-up procedures such as extraction and off-line derivatisation are eliminated and throughput of samples is significantly enhanced." @default.
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- W58161231 date "2008-02-01" @default.
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- W58161231 title "Enantioselective gas chromatographic analysis of aqueous samples by on-line derivatisation" @default.
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- W58161231 doi "https://doi.org/10.1016/j.chroma.2007.12.069" @default.
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