Matches in SemOpenAlex for { <https://semopenalex.org/work/W581854629> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W581854629 abstract "Nitric oxide is a free-radical gas which can exert both protective and damaging effects. The objectives of the thesis were: (i) to investigate arginine metabolism in isolated rat gastric mucosal cells, (ii) to investigate the role of NO in the induction of ornithine decarboxylase in the rat gastric mucosa damaged by hypertonic saline in vivo, (iii) to expose primary cultures of guinea-pig gastric mucosal cells to oxidative challenge and an NO donor, and to investigate the response in terms of heat shock protein 72 (HSP 72) induction, and (iv) to investigate the induction of iNOS and the role of potential modulators of activity in gastric cell lines. Isolated rat gastric mucosal cells converted exogenous arginine to ornithine and citrulline. This metabolism of arginine was not affected by a range of NO synthase inhibitors, but was reduced by the arginase inhibitors NG-hydroxy-L-arginine and L-ornithine. Thus, the predominant pathway of arginine metabolism involves arginase and ornithine transcarbamoylase, not NO synthase. Pretreatment of rats with NG-nitro-L-arginine promoted activation of ornithine decarboxylase after intragastric hypertonic saline, but did not increase acid phosphatase release (damage). NO may therefore restrict activation of ornithine decarboxylase in response to damage. Exposure of primary cultures of guinea-pig gastric mucosal cells to S-nitroso-N-acetyl-penicillamine (SNAP) caused a concentration dependent induction of HSP 72, which was inhibited by an NO scavenger and blockade of transcription. The effect of SNAP was enhanced by decreasing the intracellular reduced thiol content with diethyl maleate, which itself also induced HSP 72 formation. Substantial amounts of NO may induce defensive responses in cells. Induction of iNOS was not detected in HGT-1 or AGS cells exposed to cytokines. Conclusions An arginase pathway may restrict availability of arginine for NO synthase in gastric mucosa or may be present to supply ornithine for polyamine synthesis. NO may modulate the response to damage of the stomach epithelium in vivo. Exogenous NO may induce a defensive response in gastric mucosal cells." @default.
- W581854629 created "2016-06-24" @default.
- W581854629 creator A5047815568 @default.
- W581854629 date "1998-05-01" @default.
- W581854629 modified "2023-09-27" @default.
- W581854629 title "Generation of nitric oxide and its protective and toxic actions in the gastrointestinal tract" @default.
- W581854629 hasPublicationYear "1998" @default.
- W581854629 type Work @default.
- W581854629 sameAs 581854629 @default.
- W581854629 citedByCount "0" @default.
- W581854629 crossrefType "dissertation" @default.
- W581854629 hasAuthorship W581854629A5047815568 @default.
- W581854629 hasConcept C121289238 @default.
- W581854629 hasConcept C134018914 @default.
- W581854629 hasConcept C164007495 @default.
- W581854629 hasConcept C181199279 @default.
- W581854629 hasConcept C185592680 @default.
- W581854629 hasConcept C2776796294 @default.
- W581854629 hasConcept C2777468819 @default.
- W581854629 hasConcept C2777622882 @default.
- W581854629 hasConcept C2779129087 @default.
- W581854629 hasConcept C2779422922 @default.
- W581854629 hasConcept C2781443798 @default.
- W581854629 hasConcept C515207424 @default.
- W581854629 hasConcept C519581460 @default.
- W581854629 hasConcept C55493867 @default.
- W581854629 hasConcept C86803240 @default.
- W581854629 hasConcept C98274493 @default.
- W581854629 hasConceptScore W581854629C121289238 @default.
- W581854629 hasConceptScore W581854629C134018914 @default.
- W581854629 hasConceptScore W581854629C164007495 @default.
- W581854629 hasConceptScore W581854629C181199279 @default.
- W581854629 hasConceptScore W581854629C185592680 @default.
- W581854629 hasConceptScore W581854629C2776796294 @default.
- W581854629 hasConceptScore W581854629C2777468819 @default.
- W581854629 hasConceptScore W581854629C2777622882 @default.
- W581854629 hasConceptScore W581854629C2779129087 @default.
- W581854629 hasConceptScore W581854629C2779422922 @default.
- W581854629 hasConceptScore W581854629C2781443798 @default.
- W581854629 hasConceptScore W581854629C515207424 @default.
- W581854629 hasConceptScore W581854629C519581460 @default.
- W581854629 hasConceptScore W581854629C55493867 @default.
- W581854629 hasConceptScore W581854629C86803240 @default.
- W581854629 hasConceptScore W581854629C98274493 @default.
- W581854629 hasLocation W5818546291 @default.
- W581854629 hasOpenAccess W581854629 @default.
- W581854629 hasPrimaryLocation W5818546291 @default.
- W581854629 hasRelatedWork W1501833360 @default.
- W581854629 hasRelatedWork W1711251896 @default.
- W581854629 hasRelatedWork W1761071577 @default.
- W581854629 hasRelatedWork W1966051238 @default.
- W581854629 hasRelatedWork W1967574915 @default.
- W581854629 hasRelatedWork W1976571089 @default.
- W581854629 hasRelatedWork W2002830929 @default.
- W581854629 hasRelatedWork W2025861036 @default.
- W581854629 hasRelatedWork W2026403400 @default.
- W581854629 hasRelatedWork W2058220747 @default.
- W581854629 hasRelatedWork W2074915633 @default.
- W581854629 hasRelatedWork W2099194124 @default.
- W581854629 hasRelatedWork W2111309960 @default.
- W581854629 hasRelatedWork W2121242925 @default.
- W581854629 hasRelatedWork W2413174399 @default.
- W581854629 hasRelatedWork W2604697020 @default.
- W581854629 hasRelatedWork W2915013247 @default.
- W581854629 hasRelatedWork W344358030 @default.
- W581854629 hasRelatedWork W66300024 @default.
- W581854629 hasRelatedWork W93299900 @default.
- W581854629 isParatext "false" @default.
- W581854629 isRetracted "false" @default.
- W581854629 magId "581854629" @default.
- W581854629 workType "dissertation" @default.