Matches in SemOpenAlex for { <https://semopenalex.org/work/W584342014> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W584342014 abstract "Alpha- and beta-tectorin (Tecta and Tectb) are major non-collagenous components ofthe tectorial membrane (TM). The presence of a zona pellucida (ZP) domain in bothtectorins suggests that Tecta and Tectb can form hetero- or homopolymers. It is unclear,however, how these proteins assemble to form the TM matrix. The mechanisms ofapical targeting, secretion and processing of the tectorins are also unexplored. I usedfluorescently-tagged tectorin constructs for stable transfection into polarised epithelialMDCK cells or transient expression in mouse cochlear cultures to develop an in vitromodel of TM matrix assembly. Significant amounts of matrix were not observed withstable tectorin expression in monolayer cultures of MDCK cells. In contrast, I observedsubstantial amounts of dense extracellular matrix on the apical surfaces of outgrowthzone cells when cochlear cultures were transiently transfected with either Tecta orTectb. When ectopically expressed in hair cells, Tecta and Tectb locate to the distal tipsof the hair bundle.To study the role of the inner-ear protein Ceacam16 in hearing, we generated aCeacam16 functional null mouse model. The Ceacam16 gene was inactivated bytargeted replacement of exons 2-5 with the bacterial lacZ gene. β-gal staining Iperformed reveals that Ceacam16 is expressed in the epithelial cells of the spiral limbusand inner sulcus, and in both the pillar cells and Deiter’s cells. I first detected the presence of Ceacam16 in the TM at P12, four days before the defined striated-sheetmatrix is observed. Transmission electron microscopy reveals a complete loss ofstriated-sheet matrix in Ceacam16 null mice in comparison to the wild-type.The results of this thesis suggest neonatal mouse cochlear cultures as a model forstudying tectorin-based extracellular matrix production and also reveal that Ceacam16 isrequired for normal formation and/or maintenance of striated-sheet matrix." @default.
- W584342014 created "2016-06-24" @default.
- W584342014 creator A5018340652 @default.
- W584342014 date "2013-11-04" @default.
- W584342014 modified "2023-09-23" @default.
- W584342014 title "In vitro and in vivo analysis of the assembly of the non-collagenous tectorial membrane matrix" @default.
- W584342014 hasPublicationYear "2013" @default.
- W584342014 type Work @default.
- W584342014 sameAs 584342014 @default.
- W584342014 citedByCount "0" @default.
- W584342014 crossrefType "dissertation" @default.
- W584342014 hasAuthorship W584342014A5018340652 @default.
- W584342014 hasConcept C104317684 @default.
- W584342014 hasConcept C105702510 @default.
- W584342014 hasConcept C106487976 @default.
- W584342014 hasConcept C153911025 @default.
- W584342014 hasConcept C185592680 @default.
- W584342014 hasConcept C189165786 @default.
- W584342014 hasConcept C2778500370 @default.
- W584342014 hasConcept C2781459932 @default.
- W584342014 hasConcept C43617362 @default.
- W584342014 hasConcept C54009773 @default.
- W584342014 hasConcept C55493867 @default.
- W584342014 hasConcept C86803240 @default.
- W584342014 hasConcept C95444343 @default.
- W584342014 hasConceptScore W584342014C104317684 @default.
- W584342014 hasConceptScore W584342014C105702510 @default.
- W584342014 hasConceptScore W584342014C106487976 @default.
- W584342014 hasConceptScore W584342014C153911025 @default.
- W584342014 hasConceptScore W584342014C185592680 @default.
- W584342014 hasConceptScore W584342014C189165786 @default.
- W584342014 hasConceptScore W584342014C2778500370 @default.
- W584342014 hasConceptScore W584342014C2781459932 @default.
- W584342014 hasConceptScore W584342014C43617362 @default.
- W584342014 hasConceptScore W584342014C54009773 @default.
- W584342014 hasConceptScore W584342014C55493867 @default.
- W584342014 hasConceptScore W584342014C86803240 @default.
- W584342014 hasConceptScore W584342014C95444343 @default.
- W584342014 hasLocation W5843420141 @default.
- W584342014 hasOpenAccess W584342014 @default.
- W584342014 hasPrimaryLocation W5843420141 @default.
- W584342014 hasRelatedWork W1254193995 @default.
- W584342014 hasRelatedWork W1533562976 @default.
- W584342014 hasRelatedWork W1572562527 @default.
- W584342014 hasRelatedWork W1981088192 @default.
- W584342014 hasRelatedWork W1985582672 @default.
- W584342014 hasRelatedWork W2035495803 @default.
- W584342014 hasRelatedWork W2038609878 @default.
- W584342014 hasRelatedWork W2038676165 @default.
- W584342014 hasRelatedWork W2041271050 @default.
- W584342014 hasRelatedWork W2055693021 @default.
- W584342014 hasRelatedWork W2072885547 @default.
- W584342014 hasRelatedWork W2134927101 @default.
- W584342014 hasRelatedWork W2141078288 @default.
- W584342014 hasRelatedWork W2143340944 @default.
- W584342014 hasRelatedWork W2397129617 @default.
- W584342014 hasRelatedWork W2413010514 @default.
- W584342014 hasRelatedWork W2416914556 @default.
- W584342014 hasRelatedWork W2792908392 @default.
- W584342014 hasRelatedWork W2928726775 @default.
- W584342014 hasRelatedWork W3155918349 @default.
- W584342014 isParatext "false" @default.
- W584342014 isRetracted "false" @default.
- W584342014 magId "584342014" @default.
- W584342014 workType "dissertation" @default.