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- W58444103 abstract "Growth profiles of the batch and fed-batch culture of hybridoma cells producing monoclonal antibody were simulated using a structured model previously developed. The model describes the production of cellular macromolecules and monoclonal antibody, the metabolism of glucose and glutamine with the production of lactate and ammonia, and the profiles of cell growth in batch and fed-batch culture. Equations describing the cells arrested in G1 phase (Linardos et al., 1992; Suzuki et al., 1989) was included in this model to describe the increase of the specific antibody productivity in the near-zero specific growth rate, which was observed in the recent experiments in fed-batch cultures of a previous study. This model well predicted the increase ofspecific antibody production rate and the decline of the specific production rate of cellular macromolecules such as DNA, RNA, protein, and polysaccharide with lower specific growth rate in the fed-batch culture." @default.
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- W58444103 date "2005-11-30" @default.
- W58444103 modified "2023-10-18" @default.
- W58444103 title "A Kinetic Model of Macromolecular Metabolism in Batch and Fed-Batch Cultures of Hybridoma Cells Producing Monoclonal Antibody" @default.
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- W58444103 doi "https://doi.org/10.1007/0-306-46869-7_13" @default.
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