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- W585024704 abstract "Ramie [Boehmeria nivea L. (Guad.)] is a perennial herbaceous plant of the urticaceae family. Ramie can be affected by many insect pests above and below the ground during the growing season. The pests damage has become an important factor affecting ramie yield and quality in China. Application of chemical insecticides not only is less effective and costly, but also causes environ-mental pollution. Development of a genetic transformation system to introduce desired genes (e. g. toxin genes) into ramie could improve its agromonic traits such as resistance to insects. The study was conducted to transfer CryIA gene and CpT1 gene into the ramie variety Zhongzhu 1. The CryIA gene encodes Bt toxin protein from Bacillus thuringiensis toxin of Vitis pseudoreticulata; while, the CpT1 gene encodes a cowpea trypsin inhibitor produced by Vigna unguiculata. Synthesized CryIA gene and CpT1 gene were inserted into plant expression vector pGBI4ABC which carried high efficient bivalent insect resistant genes. With high frequency of adventitious shoot induction and plant regeneration, ramie hypocotyls of the variety Zhongzhu 1 were transformed with Agrobacterium tumefaciens strain LB4404 harboring the plant expression vector pGBI4ABC, and then shoot buds formed from calli induced from hypocotyls were selected with the mixed solution of 50 mg L^(-1) Kanamycin and 300 mg L^(-1) Cefotaxime. The result showed that 32 Kanamycin-resistant plants were obtained, and therein 26 plants were positive in PCR test. indicating that 80% of the Kanamycin-resistant plants contain CryIA gene and CpT1 gene. Southern blot and GUS analysis also verified that the foreign genes of CryIA and CpT1 were successfully integrated into the genome of the transgenic plants and expressed." @default.
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- W585024704 date "2010-05-14" @default.
- W585024704 modified "2023-09-26" @default.
- W585024704 title "Transgenic of Ramie with Synthetic <I>CryIA</I>+<I>CpTI</I> Gene by <I>Agrobacterium tumefaciens</I>-Mediated" @default.
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- W585024704 doi "https://doi.org/10.3724/sp.j.1006.2010.00788" @default.
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