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• W58511419 abstract "Iodine-125 radioprobing is based on incorporation of radioiodine into a defined position in a nucleic acid molecule. Decay of (125)I results in the emission of multiple, low-energy Auger electrons that, along with positively charged residual daughter nuclide, produce DNA strand breaks. The probability of such strand breaks at a given nucleotide is in inverse proportion to the distance from the (125)I atom to the sugar of that nucleotide. Therefore, conclusions can be drawn about the conformation or folding of a DNA or RNA molecule based on the distribution of (125)I decay-induced strand breaks. Here we describe in detail the application (125)I radioprobing for studying the conformation of quadruplex structures, and discuss the advantages and limitations of the method." @default.
• W58511419 created "2016-06-24" @default.
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• W58511419 date "2009-10-23" @default.
• W58511419 modified "2023-09-23" @default.
• W58511419 title "Assessing DNA Structures with 125I Radioprobing" @default.
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• W58511419 doi "https://doi.org/10.1007/978-1-59745-363-9_9" @default.
• W58511419 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3406406" @default.
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