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- W58641390 abstract "An unstructured mathematical model has been developed in our laboratory to describe the growth and infection of sf9 insect cells by a recombinant Baculovirus (pAc-360 β-Gal). The model quantitatively predicts the effects of altering the three key optimisation parameters, time of infection (TOI), multiplicity of infection (MOI) and time of harvest (TOH), in two different media, IPL-41+5% FCS+0.1% Pluronic F68 and Sf900II (Gibco). Here, the model is described for the limiting case of infection of sf9 cells in IPL-41+5% FCS+0.1% Pluronic F68 medium with a high multiplicity of infection, ie. the case of synchronous infection. Using parameters obtained from a series of independent experiments, it will be shown that the model quantitatively describes the trade off between cell and product yield observed at high cell density by depletion of a limiting substrate." @default.
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- W58641390 date "1994-01-01" @default.
- W58641390 modified "2023-09-27" @default.
- W58641390 title "Modelling insect cell cultures infected with recombinant baculovirus" @default.
- W58641390 doi "https://doi.org/10.1016/b978-0-7506-1845-8.50121-6" @default.
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