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- W587062864 abstract "目的 通过构建Cav1．3基因K228E突变，观察K228E突变对L-型钙通道电生理特性及蛋白表达和转运的影响。确定钙通道α1亚基结构域IS4段上N-端带正电荷的氨基酸对该通道电生理学特性的作用，从而为创建钙通道起搏器奠定基础。方法①一步法快速定点诱变构建Cav1．3基因K228E突变体的真核表达载体；②膜片钳观察突变钙通道电流，激光共聚焦技术观察突变钙通道蛋白在细胞内的表达和定位。结果①检测表达于HEK293细胞上的L-型钙电流：转染野生型Cav1．3质粒和相关亚基质粒，可检出L型钙电流，其半激活电压V1/2=（-36．56±2．08）mV、半失活电压V1／2=（-42．93±0．14）mV（5mmol Ba^2＋）；而转染K228E-Cavl．3质粒和相关亚基质粒的HEK293T细胞上未检测到钙电流；②检测K228E突变通道蛋白在细胞内的表达和定位：转染野生型和突变型Car1．3质粒及相关亚基质粒，48h后观察到野生型与突变型钙通道蛋白在细胞膜上都有表达。结论Cav1．3基因K228E突变虽不影响L-型钙通道蛋白质的表达与转运，但却不表达钙通道电流，是一种功能丧失性突变。" @default.
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- W587062864 date "2009-01-01" @default.
- W587062864 modified "2023-09-23" @default.
- W587062864 title "Cav1．3L-型钙通道K228E突变体的构建及功能研究" @default.
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