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- W58733997 abstract "Abstract L-selectin mediates tethering and rolling of lymphocytes in high endothelial venules. To study the transcriptional regulation of L-selectin, we cloned 5′-flanking sequences of the murine L-selectin gene 1084 bp upstream of the translational start site. Analysis of the 5′-flanking sequence shows no TATA box, but an Initiator-like element present around position −104. Multiple transcription initiation sites were mapped between −119 and −15 by 5′RACE. Transfection of serially truncated 5′-flanking regions linked to a reporter gene in Jurkat and EL-4 cells identified that nucleotides −2 to −284 contained the elements to drive high level gene expression. Transfection of the constructs into COS 7 cells, which lacks L-seletin, showed negligible luciferase activity. Within this region we identified several cis-elements, including IRF-2, Sp1, Ets-1, MZF-1. Site-directed mutagenesis of the putative binding sites showed that both Sp1 and Ets binding sites were positive regulatory elements. EMSA and CHIP confirmed the specific binding of Sp1, MZF-1, and Ets1. These results provide a molecular basis for further investigation of the involvement of L-selectin in regulating lymphocyte tethering and rolling." @default.
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- W58733997 date "2007-04-01" @default.
- W58733997 modified "2023-09-27" @default.
- W58733997 title "Transcriptional regulation of L-selectin (35.19)" @default.
- W58733997 doi "https://doi.org/10.4049/jimmunol.178.supp.35.19" @default.
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