FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W588646564> ?p ?o ?g. }

• W588646564 endingPage "e0129229" @default.
• W588646564 startingPage "e0129229" @default.
• W588646564 abstract "To explore the conservation of Src homology 3 (SH3) domain-mediated networks in evolution, we compared the specificity landscape of these domains among four yeast species, Saccharomyces cerevisiae, Ashbya gossypii, Candida albicans, and Schizosaccharomyces pombe, encompassing 400 million years of evolution. We first aligned and catalogued the families of SH3-containing proteins in these four species to determine the relationships between homologous domains. Then, we tagged and purified all soluble SH3 domains (82 in total) to perform a quantitative peptide assay (SPOT) for each SH3 domain. All SPOT readouts were hierarchically clustered and we observed that the organization of the SH3 specificity landscape in three distinct profile classes remains conserved across these four yeast species. We also produced a specificity profile for each SH3 domain from manually aligned top SPOT hits and compared the within-family binding motif consensus. This analysis revealed a striking example of binding motif divergence in a C. albicans Rvs167 paralog, which cannot be explained by overall SH3 sequence or interface residue divergence, and we validated this specificity change with a yeast two-hybrid (Y2H) assay. In addition, we show that position-weighted matrices (PWM) compiled from SPOT assays can be used for binding motif screening in potential binding partners and present cases where motifs are either conserved or lost among homologous SH3 interacting proteins. Finally, by comparing pairwise SH3 sequence identity to binding profile correlation we show that for ~75% of all analyzed families the SH3 specificity profile was remarkably conserved over a large evolutionary distance. Thus, a high sequence identity within an SH3 domain family predicts conserved binding specificity, whereas divergence in sequence identity often coincided with a change in binding specificity within this family. As such, our results are important for future studies aimed at unraveling complex specificity networks of peptide recognition domains in higher eukaryotes, including mammals." @default.
• W588646564 created "2016-06-24" @default.
• W588646564 creator A5003726801 @default.
• W588646564 creator A5013673202 @default.
• W588646564 creator A5018532546 @default.
• W588646564 creator A5024824420 @default.
• W588646564 creator A5026314869 @default.
• W588646564 creator A5034686039 @default.
• W588646564 creator A5065510179 @default.
• W588646564 creator A5074662571 @default.
• W588646564 creator A5080040899 @default.
• W588646564 creator A5080707475 @default.
• W588646564 creator A5080935038 @default.
• W588646564 creator A5091352425 @default.
• W588646564 date "2015-06-11" @default.
• W588646564 modified "2023-10-09" @default.
• W588646564 title "Evolution of the SH3 Domain Specificity Landscape in Yeasts" @default.
• W588646564 cites W1522400918 @default.
• W588646564 cites W1528221774 @default.
• W588646564 cites W1892578325 @default.
• W588646564 cites W1944802867 @default.
• W588646564 cites W1963644326 @default.
• W588646564 cites W1966419893 @default.
• W588646564 cites W1988893103 @default.
• W588646564 cites W1989455133 @default.
• W588646564 cites W1992140809 @default.
• W588646564 cites W2004900153 @default.
• W588646564 cites W2008116827 @default.
• W588646564 cites W2017783243 @default.
• W588646564 cites W2022975161 @default.
• W588646564 cites W2024812560 @default.
• W588646564 cites W2028275047 @default.
• W588646564 cites W2029818294 @default.
• W588646564 cites W2030900544 @default.
• W588646564 cites W2042950897 @default.
• W588646564 cites W2049505636 @default.
• W588646564 cites W2052315005 @default.
• W588646564 cites W2053527054 @default.
• W588646564 cites W2055043387 @default.
• W588646564 cites W2061078544 @default.
• W588646564 cites W2068386918 @default.
• W588646564 cites W2069136535 @default.
• W588646564 cites W2077524837 @default.
• W588646564 cites W2079602915 @default.
• W588646564 cites W2081933515 @default.
• W588646564 cites W2086660631 @default.
• W588646564 cites W2088362724 @default.
• W588646564 cites W2091084370 @default.
• W588646564 cites W2092544711 @default.
• W588646564 cites W2096648845 @default.
• W588646564 cites W2099769257 @default.
• W588646564 cites W2099982459 @default.
• W588646564 cites W2101231992 @default.
• W588646564 cites W2103887971 @default.
• W588646564 cites W2107982421 @default.
• W588646564 cites W2108586733 @default.
• W588646564 cites W2109178854 @default.
• W588646564 cites W2109695541 @default.
• W588646564 cites W2110311701 @default.
• W588646564 cites W2111750583 @default.
• W588646564 cites W2116117181 @default.
• W588646564 cites W2116402707 @default.
• W588646564 cites W2116905253 @default.
• W588646564 cites W2120772351 @default.
• W588646564 cites W2130974873 @default.
• W588646564 cites W2137104501 @default.
• W588646564 cites W2140996283 @default.
• W588646564 cites W2141885858 @default.
• W588646564 cites W2144362290 @default.
• W588646564 cites W2149896027 @default.
• W588646564 cites W2153283265 @default.
• W588646564 cites W2156649864 @default.
• W588646564 cites W2158133029 @default.
• W588646564 cites W2159618732 @default.
• W588646564 cites W2159624870 @default.
• W588646564 cites W2162321806 @default.
• W588646564 cites W2165862290 @default.
• W588646564 cites W2166581533 @default.
• W588646564 cites W2171572138 @default.
• W588646564 cites W2172265211 @default.
• W588646564 cites W2318867253 @default.
• W588646564 cites W2412454327 @default.
• W588646564 cites W4210623056 @default.
• W588646564 cites W4235326831 @default.
• W588646564 doi "https://doi.org/10.1371/journal.pone.0129229" @default.
• W588646564 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4466140" @default.
• W588646564 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26068101" @default.
• W588646564 hasPublicationYear "2015" @default.
• W588646564 type Work @default.
• W588646564 sameAs 588646564 @default.
• W588646564 citedByCount "7" @default.
• W588646564 countsByYear W5886465642016 @default.
• W588646564 countsByYear W5886465642017 @default.
• W588646564 countsByYear W5886465642018 @default.
• W588646564 countsByYear W5886465642019 @default.
• W588646564 countsByYear W5886465642022 @default.
• W588646564 countsByYear W5886465642023 @default.
• W588646564 crossrefType "journal-article" @default.

• next