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- W588850367 abstract "MicroRNAs are ~20-22nt non protein-coding RNA regulatory genes that posttranscriptionallyregulate many protein–coding genes to influence critical biological andmetabolic processes. While the number of known microRNA is increasing, there is nopublished data for microRNA in freshwater prawns (Macrobrachium rosenbergii) acommercially cultured and economically important food species. Through deep parallelsequencing and an in silico data analysis approach, 327 miRNA families have beenidentified from small RNA libraries with reference to both the de novo transcriptome ofM.rosenbergii obtained from RNA-Seq and to miRBase (Release 18.0, November 2011).Based on the identified mature miRNA and recovered precursor sequences that formappropriate hairpin structures, three conserved miRNA (miR125, miR750, miR993) and27 novel miRNA candidates encoding messenger-like non-coding RNA have beenidentified. miR-125, miR-750, G-m0002/H-m0009, G-m0005, G-m0008/H-m0016, Gm0011/H-m0027 and G-m0015 were selected for experimental validation with stemloopquantitative RT-PCR and were found coherent with the expression profile of deepsequencing data as evaluated with Pearson’s correlation coefficient (r = 0.835178 formiRNA in gill, r = 0.724131 for miRNA in hepatopancreas). Using a combinatorialapproach of pathway enrichment analysis and inverse expression relationship of miRNAand mRNA, four co-expressed novel miRNA candidates (G-m0005, G-m0008/H-m0016,G-m0011/H-m0027, and G-m0015) were found to be associated with energymetabolism. In addition, the expression of the three novel miRNA candidates (G-m0005,G-m0008/H-m0016, and G-m0011/H-m0027) were also found to be significantlyreduced at the 9 and 24 hours post infection in a controlled experiment of M.rosenbergiichallenged with infectious hypodermal and haematopoietic necrosis virus. Thesefindings provide a reference point to further improving the understanding of therepertoire of crustacean miRNAs." @default.
- W588850367 created "2016-06-24" @default.
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- W588850367 date "2012-01-01" @default.
- W588850367 modified "2023-09-27" @default.
- W588850367 title "Identification and characterizationof micro-RNA in Macrobrachium rosenbergii / Tan Tian Tian" @default.
- W588850367 hasPublicationYear "2012" @default.
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