Matches in SemOpenAlex for { <https://semopenalex.org/work/W591234322> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W591234322 endingPage "155" @default.
- W591234322 startingPage "151" @default.
- W591234322 abstract "All the major classes of the serum lipoproteins may be precipitated with sulfated polysaccharides and divalent cations.Burstein, et al. (1972) have shown that at neutral pH and in the presence of Ca++, Mg++ and heparin, lipoproteins can be precipitated from human serum. Methods have been developed for the isolation of the different classes of lipoproteins, i. e., low density lipoprotein (LDL), very low density lipoprotein (VLDL), and chylomicron by the stepwise addition of the reagents and increasing the ionic strength by the addition of sodium chloride.We developed quantitative precipitation method using heparin and CaCl2 solution by establishment of the standard solution, based on comparison between precipitation and ultracentrifugation methods. Quantitative estimation of the individual lipoprotein classes provides major advantages over other simplified techniques for various hyperlipoproteinemias.1) Heparin-Ca++ precipitation methodi) Reagents: (I) 0.005% heparin in 0.05M CaCl2 solution, (II) 0.005% heparin and 0.6% NaCl in 0.05M CaCl2 solution, and (III) 0.005% heparin and 0.9% NaCl in 0.05M CaCl2 solution.ii) Methods: 4ml of each solution is added to each of three tubes. To each added is 0.1ml of serum, respectively. The tubes are mixed and after 15min. at room temperature, turbidity is measured at 650nm. Turbidity in tube No. 1 represents the sum of chylomicrons, VLDL and LDL. Tube No. 2 turbidity corresponds to chylomicrons plus VLDL and tube No. 3 turbidity to chylomicron alone.2) Quantitative ultracentrifugationVLDL fraction is prepared by ultracentrifugation at 105, 400×G at 20°C for 25hrs. The LDL plus VLDL fraction is prepared form the serum-NaCl mixture with the density of 1.1315+0.0005, by ultracentrifugation at 105, 400×G at 20°C for 16hrs. Each lipoprotein fraction weighed after desiccation at 60°C and also after dry asking at 600°C, respectively. From the difference of both weights, the amount of each lipoprotein fraction is calculated by the established formula.3) Correlation between heparin-Ca++, and ultracentrifugation methodThe correlation coefficient of the LDL plus VLDL fraction for the two methods was 0.75 and the regression line was Y=0.38X-9.8, and VLDL fraction for both methods giving a correlation coefficient of 0.82 and the regression line of Y=0.33X-19.4.4) Preparation of standard solutionBecause the standard lipoprotein solutions can not be obtained, the adequate latex emulsion has been prepared by the correlation between heparin-CaCl2 and ultracentrifugation method." @default.
- W591234322 created "2016-06-24" @default.
- W591234322 creator A5009335428 @default.
- W591234322 creator A5088165428 @default.
- W591234322 date "1976-01-01" @default.
- W591234322 modified "2023-10-04" @default.
- W591234322 title "Clinical Aspects of Quantitative Serum Lipoprotein Fractionation by The Heparin-Ca<sup>++</sup> Precipitation Method" @default.
- W591234322 cites W2614133439 @default.
- W591234322 doi "https://doi.org/10.5551/jat1973.4.2_151" @default.
- W591234322 hasPublicationYear "1976" @default.
- W591234322 type Work @default.
- W591234322 sameAs 591234322 @default.
- W591234322 citedByCount "2" @default.
- W591234322 crossrefType "journal-article" @default.
- W591234322 hasAuthorship W591234322A5009335428 @default.
- W591234322 hasAuthorship W591234322A5088165428 @default.
- W591234322 hasBestOaLocation W5912343221 @default.
- W591234322 hasConcept C107054158 @default.
- W591234322 hasConcept C111368507 @default.
- W591234322 hasConcept C11772777 @default.
- W591234322 hasConcept C121332964 @default.
- W591234322 hasConcept C126322002 @default.
- W591234322 hasConcept C127313418 @default.
- W591234322 hasConcept C153294291 @default.
- W591234322 hasConcept C178790620 @default.
- W591234322 hasConcept C179104552 @default.
- W591234322 hasConcept C184651966 @default.
- W591234322 hasConcept C185592680 @default.
- W591234322 hasConcept C187428577 @default.
- W591234322 hasConcept C2777557582 @default.
- W591234322 hasConcept C2778163477 @default.
- W591234322 hasConcept C2778382381 @default.
- W591234322 hasConcept C2780072125 @default.
- W591234322 hasConcept C43617362 @default.
- W591234322 hasConcept C54689828 @default.
- W591234322 hasConcept C55493867 @default.
- W591234322 hasConcept C64016661 @default.
- W591234322 hasConcept C71924100 @default.
- W591234322 hasConcept C8243546 @default.
- W591234322 hasConceptScore W591234322C107054158 @default.
- W591234322 hasConceptScore W591234322C111368507 @default.
- W591234322 hasConceptScore W591234322C11772777 @default.
- W591234322 hasConceptScore W591234322C121332964 @default.
- W591234322 hasConceptScore W591234322C126322002 @default.
- W591234322 hasConceptScore W591234322C127313418 @default.
- W591234322 hasConceptScore W591234322C153294291 @default.
- W591234322 hasConceptScore W591234322C178790620 @default.
- W591234322 hasConceptScore W591234322C179104552 @default.
- W591234322 hasConceptScore W591234322C184651966 @default.
- W591234322 hasConceptScore W591234322C185592680 @default.
- W591234322 hasConceptScore W591234322C187428577 @default.
- W591234322 hasConceptScore W591234322C2777557582 @default.
- W591234322 hasConceptScore W591234322C2778163477 @default.
- W591234322 hasConceptScore W591234322C2778382381 @default.
- W591234322 hasConceptScore W591234322C2780072125 @default.
- W591234322 hasConceptScore W591234322C43617362 @default.
- W591234322 hasConceptScore W591234322C54689828 @default.
- W591234322 hasConceptScore W591234322C55493867 @default.
- W591234322 hasConceptScore W591234322C64016661 @default.
- W591234322 hasConceptScore W591234322C71924100 @default.
- W591234322 hasConceptScore W591234322C8243546 @default.
- W591234322 hasIssue "2" @default.
- W591234322 hasLocation W5912343221 @default.
- W591234322 hasOpenAccess W591234322 @default.
- W591234322 hasPrimaryLocation W5912343221 @default.
- W591234322 hasRelatedWork W1027509084 @default.
- W591234322 hasRelatedWork W1422364393 @default.
- W591234322 hasRelatedWork W1583749263 @default.
- W591234322 hasRelatedWork W1972043516 @default.
- W591234322 hasRelatedWork W2103972341 @default.
- W591234322 hasRelatedWork W2166773426 @default.
- W591234322 hasRelatedWork W2396370662 @default.
- W591234322 hasRelatedWork W2434523491 @default.
- W591234322 hasRelatedWork W50315368 @default.
- W591234322 hasRelatedWork W73532347 @default.
- W591234322 hasVolume "4" @default.
- W591234322 isParatext "false" @default.
- W591234322 isRetracted "false" @default.
- W591234322 magId "591234322" @default.
- W591234322 workType "article" @default.