Matches in SemOpenAlex for { <https://semopenalex.org/work/W59132034> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W59132034 endingPage "8" @default.
- W59132034 startingPage "71" @default.
- W59132034 abstract "Previous studies in our laboratory have shown that activated eosinophils and eosinophilic cell lines inhibit the in vitro growth of MCF-7 breast tumor cells. We have also shown that IL-4 and IL-5 partially inhibit MCF-7 growth in vitro. In this study MCF-7 multicellular tumor spheroids (MTS) were developed to study the effect of eosinophils and IL-4 on tumor growth.Hypo- and hyperdense metrizamide density gradient fractions of eosinophils from peripheral blood of individuals with mild to moderate esoinophilia were co-cultured with 2-day-old MCF-7 MTS in medium containing bacto agar overlay at 37 degrees C.Light microscopic analyses revealed the attachment of eosinophil effector cells to the spheroid borders. Moreover, the culture media was greater than 90% devoid of effector cells. At six days post co-culture, very large spheroids were observed in both test and control dishes; however the necrotic cores in the co-cultures were more intense and larger than in the control. When MCF-7 tumor cells (1 x 10(6)) were pretreated with IL-4 at 0.5 ng/ml, there was a dramatic decrease in the number of spheroids formed.These data strongly indicate that cytokines like IL-4 and perhaps other eosinophil mediators are capable of killing and inhibiting tumor growth; they suggest that tumor infiltrating eosinophils can degranulate and release toxic inhibitory factors into the tumor milieu which destroy the surrounding tumor. These observations, along with the use of the eosinophil: MTS tumor model provide a unique model system for in depth studies of the role of eosinophils and the cytokines they produce in breast cancer and may offer potential therapeutic implications." @default.
- W59132034 created "2016-06-24" @default.
- W59132034 creator A5005424587 @default.
- W59132034 creator A5007423727 @default.
- W59132034 creator A5018705269 @default.
- W59132034 creator A5037758115 @default.
- W59132034 creator A5052194156 @default.
- W59132034 creator A5054430493 @default.
- W59132034 creator A5067578637 @default.
- W59132034 creator A5071416458 @default.
- W59132034 date "2003-04-12" @default.
- W59132034 modified "2023-09-26" @default.
- W59132034 title "Activated eosinophils infiltrate MCF-7 breast multicellular tumor spheroids." @default.
- W59132034 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12680196" @default.
- W59132034 hasPublicationYear "2003" @default.
- W59132034 type Work @default.
- W59132034 sameAs 59132034 @default.
- W59132034 citedByCount "3" @default.
- W59132034 countsByYear W591320342018 @default.
- W59132034 countsByYear W591320342022 @default.
- W59132034 crossrefType "journal-article" @default.
- W59132034 hasAuthorship W59132034A5005424587 @default.
- W59132034 hasAuthorship W59132034A5007423727 @default.
- W59132034 hasAuthorship W59132034A5018705269 @default.
- W59132034 hasAuthorship W59132034A5037758115 @default.
- W59132034 hasAuthorship W59132034A5052194156 @default.
- W59132034 hasAuthorship W59132034A5054430493 @default.
- W59132034 hasAuthorship W59132034A5067578637 @default.
- W59132034 hasAuthorship W59132034A5071416458 @default.
- W59132034 hasConcept C142724271 @default.
- W59132034 hasConcept C175369904 @default.
- W59132034 hasConcept C185592680 @default.
- W59132034 hasConcept C202751555 @default.
- W59132034 hasConcept C203014093 @default.
- W59132034 hasConcept C2776042228 @default.
- W59132034 hasConcept C2777037409 @default.
- W59132034 hasConcept C502942594 @default.
- W59132034 hasConcept C54355233 @default.
- W59132034 hasConcept C55493867 @default.
- W59132034 hasConcept C71924100 @default.
- W59132034 hasConcept C81885089 @default.
- W59132034 hasConcept C86803240 @default.
- W59132034 hasConceptScore W59132034C142724271 @default.
- W59132034 hasConceptScore W59132034C175369904 @default.
- W59132034 hasConceptScore W59132034C185592680 @default.
- W59132034 hasConceptScore W59132034C202751555 @default.
- W59132034 hasConceptScore W59132034C203014093 @default.
- W59132034 hasConceptScore W59132034C2776042228 @default.
- W59132034 hasConceptScore W59132034C2777037409 @default.
- W59132034 hasConceptScore W59132034C502942594 @default.
- W59132034 hasConceptScore W59132034C54355233 @default.
- W59132034 hasConceptScore W59132034C55493867 @default.
- W59132034 hasConceptScore W59132034C71924100 @default.
- W59132034 hasConceptScore W59132034C81885089 @default.
- W59132034 hasConceptScore W59132034C86803240 @default.
- W59132034 hasIssue "1A" @default.
- W59132034 hasLocation W591320341 @default.
- W59132034 hasOpenAccess W59132034 @default.
- W59132034 hasPrimaryLocation W591320341 @default.
- W59132034 hasRelatedWork W1978403614 @default.
- W59132034 hasRelatedWork W1980012847 @default.
- W59132034 hasRelatedWork W2007117313 @default.
- W59132034 hasRelatedWork W2033530968 @default.
- W59132034 hasRelatedWork W2047305992 @default.
- W59132034 hasRelatedWork W2105022733 @default.
- W59132034 hasRelatedWork W2522607455 @default.
- W59132034 hasRelatedWork W3171746498 @default.
- W59132034 hasRelatedWork W403877154 @default.
- W59132034 hasRelatedWork W4248973107 @default.
- W59132034 hasVolume "23" @default.
- W59132034 isParatext "false" @default.
- W59132034 isRetracted "false" @default.
- W59132034 magId "59132034" @default.
- W59132034 workType "article" @default.