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• W59304830 abstract "Research Article1 February 1987free access Expression of retroviral vectors in transgenic mice obtained by embryo infection. C.L. Stewart C.L. Stewart Search for more papers by this author S. Schuetze S. Schuetze Search for more papers by this author M. Vanek M. Vanek Search for more papers by this author E.F. Wagner E.F. Wagner Search for more papers by this author C.L. Stewart C.L. Stewart Search for more papers by this author S. Schuetze S. Schuetze Search for more papers by this author M. Vanek M. Vanek Search for more papers by this author E.F. Wagner E.F. Wagner Search for more papers by this author Author Information C.L. Stewart, S. Schuetze, M. Vanek and E.F. Wagner The EMBO Journal (1987)6:383-388https://doi.org/10.1002/j.1460-2075.1987.tb04766.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Pre-implantation embryos were infected with the retroviral vector MMCV-neo, which carries the neomycin resistance (neo) gene and the v-myc gene. Three transgenic substrains (M-TKneo 1-3) were derived which stably transmit a single intact copy of the vector. In all of the substrains, expression of the neo gene from the internal thymidine kinase (TK) promoter was detected, with two of the substrains expressing the gene in all tissues analysed. In the third substrain, the vector had integrated on the X chromosome and neo expression varied between different tissues. A second series of transgenic mice were obtained with the retroviral vector SAX, in which the human adenosine deaminase cDNA (ADA) is under the control of an internal SV40 promoter. Four substrains (M-SAX 1-4) were analysed; however, no expression of the ADA cDNA was detected. In all mice, no expression was found of the genes under the control of the viral 5′ long terminal repeats (LTRs). In the M-TKneo substrains the vector was hypomethylated irrespective of its expression whereas in the M-SAX mice the vector was hypermethylated. These results demonstrate for the first time that the TK promoter can apparently express a gene in all tissues of adult mice and that retroviral vectors with internal promoters may provide an alternative to DNA injection for the efficient expression of genes in transgenic mice. Previous ArticleNext Article Volume 6Issue 21 February 1987In this issue RelatedDetailsLoading ..." @default.
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• W59304830 title "Expression of retroviral vectors in transgenic mice obtained by embryo infection." @default.
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